Abstract
Cell-penetrating peptides (CPPs) can facilitate uptake of quantum dots (QDs) for a variety of basic and applied sciences. Here we describe a method that utilizes simple noncovalent interactions to complex QDs and CPPs. We further describe methods to study uptake mechanisms of the QD/CPP complex. The inhibitor study coupled with the RNA interference (RNAi) technique provides a comprehensive approach to elucidate cellular entry of the QD/CPP complex.
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Acknowledgments
The authors thank Robert S. Aronstam for technical editing. This work was supported by Award No. R15EB009530 from the National Institute of Biomedical Imaging and Bioengineering (to Y.-W. Huang) and the National Science Council (NSC 97-2621-B-259-003-MY3 to H.-J. Lee), Taiwan.
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Huang, YW., Lee, HJ., Liu, B.R., Chiang, HJ., Wu, CH. (2013). Cellular Internalization of Quantum Dots. In: Weissig, V., Elbayoumi, T., Olsen, M. (eds) Cellular and Subcellular Nanotechnology. Methods in Molecular Biology, vol 991. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-336-7_23
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DOI: https://doi.org/10.1007/978-1-62703-336-7_23
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-335-0
Online ISBN: 978-1-62703-336-7
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