Abstract
In vivo transplantation of putative populations of hematopoietic stem cells (HSC) and assessment of their engraftment is considered the golden standard to assess their quality and degree of stemness. Transplantation is usually carried out by intravenous injection in murine models and assessment of engraftment is performed by monitoring the number and type of mature blood cells produced by the donor cells in time. In contrast intravenous injection of mesenchymal stem cells (MSC), the multipotent stem cells present in bone marrow and capable of differentiating to osteoblasts, chondrocytes and adipocytes, has not been successful. This is due to limited or absent engraftment levels. Here, we describe the use of intra-femoral injection as an improved method to assess MSC engraftment to bone and bone marrow and their quality.
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References
Rossi DJ et al (2005) Cell intrinsic alterations underlie hematopoietic stem cell aging. Proc Natl Acad Sci USA 102(26):9194–9199
Pittenger MF et al (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284(5411):143–147
Friedenstein AJ, Chailakhyan RK, Gerasimov UV (1987) Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers. Cell Tissue Kinet 20(3):263–272
Baxter MA et al (2004) Study of telomere length reveals rapid aging of human marrow stromal cells following in vitro expansion. Stem Cells 22(5):675–682
Daga A et al (2002) Enhanced engraftment of EPO-transduced human bone marrow stromal cells transplanted in a 3D matrix in non-Âconditioned NOD/SCID mice. Gene Ther 9(14):915–921
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Mohanty, S.T., Bellantuono, I. (2013). Intra-femoral Injection of Human Mesenchymal Stem Cells. In: Turksen, K. (eds) Stem Cells and Aging. Methods in Molecular Biology, vol 976. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-317-6_10
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DOI: https://doi.org/10.1007/978-1-62703-317-6_10
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-316-9
Online ISBN: 978-1-62703-317-6
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