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Stem Cells and Aging

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Kursad Turksen

    1. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada

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  • Includes cutting-edge methods and protocols in the MiMB standard format
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 976)

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Table of contents (13 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Comprehensive Hematopoietic Stem Cell Isolation Methods

    • Kyle Rector, Yi Liu, Gary Van Zant
    Pages 1-15

  3. Serial Transplantation of Bone Marrow to Test Self-renewal Capacity of Hematopoietic Stem Cells In Vivo

    • Charusheila Ramkumar, Rachel M. Gerstein, Hong Zhang
    Pages 17-24

  4. Mouse Hematopoietic Stem Cell Transplantation

    • Hui Cheng, Paulina H. Liang, Tao Cheng
    Pages 25-35

  5. Isolation, Characterization, and Transplantation of Adult Liver Progenitor Cells

    • Mladen I. Yovchev, Mariana D. Dabeva, Michael Oertel
    Pages 37-51

  6. Isolation of Muscle-Derived Stem/Progenitor Cells Based on Adhesion Characteristics to Collagen-Coated Surfaces

    • Mitra Lavasani, Aiping Lu, Seth D. Thompson, Paul D. Robbins, Johnny Huard, Laura J. Niedernhofer
    Pages 53-65

  7. Human Myoblasts from Skeletal Muscle Biopsies: In Vitro Culture Preparations for Morphological and Cytochemical Analyses at Light and Electron Microscopy

    • Manuela Malatesta, Marzia Giagnacovo, Rosanna Cardani, Giovanni Meola, Carlo Pellicciari
    Pages 67-79

  8. Cardiac Stem Cell Senescence

    • Daniela Cesselli, Federica D’Aurizio, Patrizia Marcon, Natascha Bergamin, Carlo Alberto Beltrami, Antonio Paolo Beltrami
    Pages 81-97

  9. Isolation of Mesenchymal Stem Cells from Human Bone and Long-Term Cultivation Under Physiologic Oxygen Conditions

    • Sebastian Klepsch, Angelika Jamnig, Daniela Trimmel, Magdalena Schimke, Werner Kapferer, Regina Brunauer et al.
    Pages 99-109

  10. Methods for Assessing Effects of Wnt/β-Catenin Signaling in Senescence of Mesenchymal Stem Cells

    • Hai-jie Wang, Yu-zhen Tan
    Pages 111-130

  11. Intra-femoral Injection of Human Mesenchymal Stem Cells

    • Sindhu T. Mohanty, Ilaria Bellantuono
    Pages 131-141

  12. Tracking of Replicative Senescence in Mesenchymal Stem Cells by Colony-Forming Unit Frequency

    • Anne Schellenberg, Hatim Hemeda, Wolfgang Wagner
    Pages 143-154

  13. Chromatin Immunoprecipitation Coupled by Quantitative Real-Time PCR as a Tool for Analyzing Epigenetic Regulation of Stem Cell Aging

    • Seunghee Lee, Ji-Won Jung, Kyung-Sun Kang
    Pages 155-165

  14. Quantitative Fluorescence In Situ Hybridization on Paraffin Embedded Tissue

    • Mario Ricciardi, Mauro Krampera, Marco Chilosi
    Pages 167-173

  15. Back Matter

    Pages 175-179
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Keywords

About this book

Over time, it has become clear that changes in stem cells do occur during aging, not only in their number but also in their relationship to their microenvironment and their functionality as reflected in changes to their metabolome.  Stem Cells and Aging: Methods and Protocols brings together chapters from expert contributors with protocols critical for exploring the biology of stem cell aging, all of which is key for understanding these age-related stem cell changes at a basic biology level and at the level of their impacts for regenerative medicine.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Concise and easy to use, Stem Cells and Aging: Methods and Protocols serves as an ideal reference to guide investigators toward further valuable answers to the problems of our aging population.

Editors and Affiliations

  • Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Stem Cells and Aging

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-317-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-316-9Published: 13 February 2013

  • Softcover ISBN: 978-1-4939-6303-4Published: 23 August 2016

  • eBook ISBN: 978-1-62703-317-6Published: 12 February 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 179

  • Topics: Cell Biology, Stem Cells, Regenerative Medicine/Tissue Engineering

  • Industry Sectors: Biotechnology, Pharma

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