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Production of Primary Human CD4+ T Cell Lines and Clones

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Antigen Processing

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 960))

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Abstract

Tetramer staining of CD4+ T cells is a valuable technique in immunology for detecting rare autoreactive T cells. Generating clones or cell lines from autoantigen tetramer-positive CD4+ T cells allows further characterization and phenotyping of autoreactive cells.

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References

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Acknowledgments

We thank Ms Kelly Geubtner, Elsa Laughlin, Nancy Danke, and Drs Erik Novak, Eddie James and William Kwok for their valuable contribution to the development of MHC class II tetramer assay and T cell cloning protocols.

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Authors and Affiliations

  1. Benaroya Research Institute, Seattle, WA, USA

    Jessica Matthis & Helena Reijonen

Authors
  1. Jessica Matthis
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  2. Helena Reijonen
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Corresponding author

Correspondence to Helena Reijonen .

Editor information

Editors and Affiliations

  1. Institut National de la Santé et de la Recherche Médicale, Unité 1013, Université Paris Descartes, Faculté de médecine, Paris, France

    Peter van Endert

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Matthis, J., Reijonen, H. (2013). Production of Primary Human CD4+ T Cell Lines and Clones. In: van Endert, P. (eds) Antigen Processing. Methods in Molecular Biology™, vol 960. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-218-6_40

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  • DOI: https://doi.org/10.1007/978-1-62703-218-6_40

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-217-9

  • Online ISBN: 978-1-62703-218-6

  • eBook Packages: Springer Protocols

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