Antigen Processing

Methods and Protocols

  • Peter van Endert

Part of the Methods in Molecular Biology™ book series (MIMB, volume 960)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Stefan Tenzer, Tobias Hain, Hendrik Berger, Hansjörg Schild
    Pages 1-14
  3. Kathrin Textoris-Taube, Christin Keller, Ulrike Kuckelkorn, Peter-M. Kloetzel
    Pages 15-29
  4. Michael Basler, Marcus Groettrup
    Pages 31-39
  5. Marlieke L. M. Jongsma, Jacques Neefjes
    Pages 53-65
  6. Pamela A. Wearsch, Peter Cresswell
    Pages 67-79
  7. David B. Guiliano, Antony N. Antoniou
    Pages 93-108
  8. Marian L. Burr, Jessica M. Boname, Paul J. Lehner
    Pages 109-125
  9. Daniëlle Horst, Maaike E. Ressing, Arend Mulder, Emmanuel J. H. J. Wiertz
    Pages 127-136
  10. Daniel J. Kowalewski, Stefan Stevanović
    Pages 145-157
  11. Nico Trautwein, Stefan Stevanović
    Pages 159-168
  12. Nathalie Vigneron, Wenbin Ma, Alexandre Michaux, Benoît J. Van den Eynde
    Pages 187-207
  13. Ayesha Murshid, Jianlin Gong, Stuart K. Calderwood
    Pages 209-217
  14. Nicholas A. Siciliano, Lan Huang, Laurence C. Eisenlohr
    Pages 219-245
  15. Ole Lund, Edita Karosiene, Claus Lundegaard, Mette Voldby Larsen, Morten Nielsen
    Pages 247-260

About this book


Antigen processing is a biological process that prepares antigens for the presentation to special cells in the immune system called T lymphocytes. In Antigen Processing: Methods and Protocols, expert researchers in the field provide a comprehensive set of protocols for studying presentation of antigens produced in the standard processing pathways for MHC class I and class II molecules. The chapters follow chronology of intracellular processing events, ending with recognition of peptide-MHC complexes at the cell surface by T lymphocytes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Antigen Processing: Methods and Protocols is designed for beginners and experts interested in studying antigen processing.


MHC class I molecules MHC class II molecules cytosolic proteases dendritic cells endogenous peptides endosomal proteases kinetics of peptide lentiviruses macroautophagy peptide-MHC

Editors and affiliations

  • Peter van Endert
    • 1
  1. 1.Institut National de la Santé et de la Recherche Médicale, Unité 1013Université Paris Descartes, Faculté de médecineParisFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-217-9
  • Online ISBN 978-1-62703-218-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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