Abstract
Routine large-scale two-dimensional (2D) crystallization trials are good candidates for automation. For imaging the large number of grids prepared from 2D crystallization trials, a two-pass imaging protocol using Leginon and a grid handling robot is described. A manual target selection bridges the two imaging passes. The two passes can be combined into one if objects of interests at different stages of trials can be reliably found using automated methods.
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Acknowledgments
This work is supported by National Institutes of Health (NIH) through the National Center for Research Resources (NCRR) P41 program (Grants RR17573 and RR023093). I would like to thank Bridget Carragher for valuable comments and editing of the manuscript.
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Cheng, A. (2013). Automated Grid Handling and Image Acquisition for Two-Dimensional Crystal Screening. In: Schmidt-Krey, I., Cheng, Y. (eds) Electron Crystallography of Soluble and Membrane Proteins. Methods in Molecular Biology, vol 955. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-176-9_16
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DOI: https://doi.org/10.1007/978-1-62703-176-9_16
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-175-2
Online ISBN: 978-1-62703-176-9
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