Electron Crystallography of Soluble and Membrane Proteins

Methods and Protocols

  • Ingeborg Schmidt-Krey
  • Yifan Cheng

Part of the Methods in Molecular Biology book series (MIMB, volume 955)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Werner Kühlbrandt
    Pages 1-16
  3. Kutti R. Vinothkumar, Patricia C. Edwards, Joerg Standfuss
    Pages 17-30
  4. Matthew C. Johnson, Tina M. Dreaden, Laura Y. Kim, Frederik Rudolph, Bridgette A. Barry, Ingeborg Schmidt-Krey
    Pages 31-58
  5. Luc Lebeau, Catherine Vénien-Bryan
    Pages 59-71
  6. Tina M. Dreaden, Maureen Metcalfe, Laura Y. Kim, Matthew C. Johnson, Bridgette A. Barry, Ingeborg Schmidt-Krey
    Pages 73-101
  7. Yoshinori Fujiyoshi
    Pages 103-118
  8. Nobuhiko Gyobu
    Pages 119-128
  9. Agustin Avila-Sakar, Xueming Li, Shawn Q. Zheng, Yifan Cheng
    Pages 129-152
  10. Marcel Arheit, Daniel Castaño-Díez, Raphaël Thierry, Bryant R. Gipson, Xiangyan Zeng, Henning Stahlberg
    Pages 171-194
  11. Marcel Arheit, Daniel Castaño-Diéz, Raphaël Thierry, Priyanka Abeyrathne, Bryant R. Gipson, Henning Stahlberg
    Pages 195-209
  12. David L. Stokes, Iban Ubarretxena-Belandia, Tamir Gonen, Andreas Engel
    Pages 273-296
  13. Marcel Arheit, Daniel Castaño-Díez, Raphaël Thierry, Bryant R. Gipson, Xiangyan Zeng, Henning Stahlberg
    Pages 313-330
  14. Deryck J. Mills, Janet Vonck
    Pages 331-351

About this book

Introduction

The basic principle of electron crystallography is to calculate a 3D density map by combining the amplitudes obtained from electron diffraction patterns with the experimental phases calculated from images of two-dimensional crystals of membrane or soluble proteins. This technology is very well developed and has produced a number of atomic models of membrane proteins in a lipid environment.  Focused on comprehensive experimental protocols, Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols covers the entire range of techniques used in electron crystallography, including protein sample preparation, 2D crystallization, and screening in negative stain over electron cryo-microscopy (cryo-EM) and data processing, as well as modeling of conformational changes. Additional chapters provide perspective on past, present, and future challenges as well as complementary methods.  Written for the popular Methods in Molecular Biology™ series, the work contains the kind of detailed descriptions and implementation advice necessary to ensure successful results.

 

Comprehensive and cutting-edge, Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols serves laboratories new to the methods as well as state-of-the-art facilities pursuing this exciting area of protein science.

Keywords

3D density map Electron cryo-cryomicroscopy Electron crystallography Negative stain Protein sample preparation Two-dimensional (2D) crystals X-ray crystallography

Editors and affiliations

  • Ingeborg Schmidt-Krey
    • 1
  • Yifan Cheng
    • 2
  1. 1.School of BiologyGeorgia Institute of TechnologyAtlantaUSA
  2. 2., Dept. Biochemistry & BiophysicsUniversity of California, San FranciscoSan FranciscoUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-176-9
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-175-2
  • Online ISBN 978-1-62703-176-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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