Abstract
Plasma membrane proteins are critical for the maintenance of biological systems and represent important targets for the treatment of disease. The hydrophobicity and low abundance of plasma membrane proteins make them difficult to analyze. The protocols given here are the efficient isolation/digestion procedures for liver plasma membrane proteomic analysis. Both protocol for the isolation of plasma membranes and protocol for the in-gel digestion of gel-embedded plasma membrane proteins are presented. The later method allows the use of a high detergent concentration to achieve efficient solubilization of hydrophobic plasma membrane proteins while avoiding interference with the subsequent LC-MS/MS analysis.
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Acknowledgements
This work was supported by National Natural Science Foundation of China (31000375 and 30770437) and grant from National 973 Project of China (2007CB914203).
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© 2012 Springer Science+Business Media New York
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Cao, R., Liang, S. (2012). Liver Plasma Membranes: An Effective Method to Analyze Membrane Proteome. In: Josic, D., Hixson, D. (eds) Liver Proteomics. Methods in Molecular Biology, vol 909. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-959-4_8
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DOI: https://doi.org/10.1007/978-1-61779-959-4_8
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-958-7
Online ISBN: 978-1-61779-959-4
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