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The Detection of NK Cell Alloreactivity by Flow Cytometric CD107a Assay

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Immunogenetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 882))

Abstract

Natural killer (NK) cell alloreactivity can be exploited in haploidentical (one haplotype mismatched) haematopoietic stem cell transplantation (HSCT) to prevent leukaemia relapse, rejection, and graft-vs-host disease (GVHD) (Blood 94:333–339; Science 295:2097–2100). If NK cell alloreactivity is to be exploited in HSCT, it is important to be able to reliably select donors who have NK alloreactivity towards the patient. The detection of donor NK alloreactivity towards patient target cells has traditionally been evaluated by NK cell cloning and 51Cr-release cytotoxicity assay. This approach is complex and time consuming with results taking up to 6 weeks. Here, we detail a novel flow cytometric CD107a-based assay capable of detecting NK cell alloreactivity in 14 days.

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Author information

Authors and Affiliations

  1. Department of Clinical Immunology, PathWest Laboratory Medicine, Royal Perth Hospital, Perth, WA, Australia

    Dianne De Santis & Campbell S. Witt

  2. Division of Hematology, Oncology and Transplantation, University of Minnesota Cancer Centre, Minneapolis, MN, USA

    Bree Foley

  3. School of Pathology and Laboratory Medicine (PaLM), University of Western Australia, Nedlands, WA, Australia

    Campbell S. Witt

  4. Department of Clinical Immunology, PathWest Laboratory Medicine, Royal Perth and Fremantle Hospitals, Perth, WA, Australia

    Frank T. Christiansen

  5. School of Pathology and Laboratory Medicine (PaLM), University of Western Australia, Perth, WA, Australia

    Frank T. Christiansen

Authors
  1. Dianne De Santis
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  2. Bree Foley
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  3. Campbell S. Witt
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  4. Frank T. Christiansen
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Corresponding author

Correspondence to Dianne De Santis .

Editor information

Editors and Affiliations

  1. PathWest Laboratory Medicine, Department of Clinical Immunology, Royal Perth Hospital, Wellington Street, Perth, 6000, Australia

    Frank T. Christiansen

  2. Australian Red Cross Blood Service, National Transplant Services, The Royal Melbourne Hospital, 150-154 Batman Street, Melbourne VIC, 3000, Australia

    Brian D. Tait

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© 2012 Springer Science+Business Media, LLC

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De Santis, D., Foley, B., Witt, C.S., Christiansen, F.T. (2012). The Detection of NK Cell Alloreactivity by Flow Cytometric CD107a Assay. In: Christiansen, F., Tait, B. (eds) Immunogenetics. Methods in Molecular Biology, vol 882. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-842-9_27

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  • DOI: https://doi.org/10.1007/978-1-61779-842-9_27

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-841-2

  • Online ISBN: 978-1-61779-842-9

  • eBook Packages: Springer Protocols

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