Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 882)
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Table of contents (34 protocols)
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Front Matter
About this book
The HLA molecules are important regulators of the immune response through mediating antigen presentation and interaction between key immune mediating cells. They are also the major histocompatibility barriers to transplantation, which is the clinical paradigm of the self versus non self concept. It is now recognized that this diverse range of gene systems involved in the control of the immune response have been shown to be important in many aspects of clinical practice. As a result many new molecular and cellular methods have been developed for identifying these genes and their polymorphisms, and immunogenetic laboratories specializing in these methods have developed to support transplantation and other clinical programs. Immunogenetics: Methods and Applications in Clinical Practice focuses on methods for human clinical practice. The emphasis rests on those assays which are of established or potential clinical utility and are likely to be included in the repertoire of tests provided by a routine diagnostic and service laboratory. This volume also contains several review chapters of the MHC complex, the KIR complex, the human immunoglobulin allotypes, as well as reviews of the methods for the detection of alloreactive NK cells and the detection of HLA antibodies by solid phase assays. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Immunogenetics: Methods and Applications in Clinical Practice seeks to serve both the immunogenetics community and the wider scientific community with a collection of detailed information and helpful tips attained by many years of experience in the field.
Reviews
From the reviews:
“This book is a comprehensive volume covering a wide-range of topics related to HLA in the clinical setting. … this book is an extremely useful reference for those working in the field of transplantation, or with an interest in the clinical applications of HLA. The introductory chapters to various topics are clear and provide a good amount of detail and are well referenced for those who wish to read further on the subject.” (Vivien Hanson, Immunology News, February, 2013)Editors and Affiliations
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PathWest Laboratory Medicine, Department of Clinical Immunology, Royal Perth Hospital, Perth, Australia
Frank T. Christiansen
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Australian Red Cross Blood Service, National Transplant Services, The Royal Melbourne Hospital, Melbourne VIC, Australia
Brian D. Tait
Bibliographic Information
Book Title: Immunogenetics
Book Subtitle: Methods and Applications in Clinical Practice
Editors: Frank T. Christiansen, Brian D. Tait
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-842-9
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2012
Hardcover ISBN: 978-1-61779-841-2Published: 06 June 2012
Softcover ISBN: 978-1-4939-5917-4Published: 23 August 2016
eBook ISBN: 978-1-61779-842-9Published: 05 June 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 689
Number of Illustrations: 135 b/w illustrations, 2 illustrations in colour
Topics: Human Genetics, Immunology
Industry Sectors: Biotechnology, Pharma