Crossmatching by Complement-Dependent Lymphocytotoxicity

Fidler, Samantha J.

doi:10.1007/978-1-61779-842-9_21

Samantha J. Fidler^3,4

Part of the book series: Methods in Molecular Biology ((MIMB,volume 882))

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Abstract

The presence of preformed donor-specific HLA antibodies detected by Complement-dependent cytotoxicity (CDC) crossmatch assay is associated with a high incidence of hyperacute or accelerated rejection and remains one of the gold standard tests pre-transplant. The standard CDC crossmatch detects IgG1, IgG3, and IgM antibody, i.e. complement fixing, bound to the native viable cell surface of lymphocytes. The crossmatch can be enhanced with the addition of anti-human-globulin to detect non-complement fixing antibodies (IgG2 and IgG4), and sensitivity can be improved with prolonged incubation times.

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References

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Authors and Affiliations

Department of Clinical Immunology, PathWest Laboratory Medicine, Royal Perth Hospital, Perth, WA, Australia
Samantha J. Fidler
School of Pathology and Laboratory Medicine (PaLM), University of Western Australia, Nedlands, WA, Australia
Samantha J. Fidler

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Samantha J. Fidler
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Correspondence to Samantha J. Fidler .

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Editors and Affiliations

PathWest Laboratory Medicine, Department of Clinical Immunology, Royal Perth Hospital, Wellington Street, Perth, 6000, Australia
Frank T. Christiansen
Australian Red Cross Blood Service, National Transplant Services, The Royal Melbourne Hospital, 150-154 Batman Street, Melbourne VIC, 3000, Australia
Brian D. Tait

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Fidler, S.J. (2012). Crossmatching by Complement-Dependent Lymphocytotoxicity. In: Christiansen, F., Tait, B. (eds) Immunogenetics. Methods in Molecular Biology, vol 882. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-842-9_21

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DOI: https://doi.org/10.1007/978-1-61779-842-9_21
Published: 26 April 2012
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-841-2
Online ISBN: 978-1-61779-842-9
eBook Packages: Springer Protocols

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