Skip to main content
SpringerLink
Log in

Biochemical and Molecular Biological Assessments of Global Cerebral Ischemia: mRNA

  • Protocol
  • First Online:
Animal Models of Acute Neurological Injuries II

Part of the book series: Springer Protocols Handbooks ((SPH))

  • 1659 Accesses

Abstract

DNA microarrays have indicated that significant gene expression changes following global cerebral ischemia occur, and that neurotherapeutic strategies may specifically impact RNA expression in ischemic cells. Thus, the assessment of specific RNA species may provide an integral aspect to understanding the molecular effects of global cerebral ischemia and therapeutic strategies. We present here a brief discussion of RNA assessments following global ischemia, and describe two of the basic techniques, northern blotting and in situ hybridization, keeping in mind the specific context of global cerebral ischemic brain injury.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Büttner F, Cordes C, Gerlach F, Heimann A, Alessandri B, Luxemburger U, Türeci O, Hankeln T, Kempski O, Burmester T (2009) Genomic response of the rat brain to global ischemia and reperfusion. Brain Res 1252: 1–14

    Article  PubMed  Google Scholar 

  2. Roberts GG, Di Loreto MJ, Marshall M, Wang J, DeGracia DJ (2007) Hippocampal cellular stress responses after global brain ischemia and reperfusion. Antioxid Redox Signal 9:2265–2275

    Article  PubMed  CAS  Google Scholar 

  3. Chen J, Nagayama T, Jin K, Stetler RA, Zhu RL, Graham SH, Simon RP (1998) Induction of caspase-3-like protease may mediate delayed neuronal death in the hippocampus after transient cerebral ischemia. J Neurosci 18: 4914–4928

    PubMed  CAS  Google Scholar 

  4. Stetler RA, Gao Y, Zukin RS, Vosler PS, Zhang L, Zhang F, Cao G, Bennett MV, Chen J (2010) Apurinic/apyrimidinic endonuclease APE1 is required for PACAP-induced neuroprotection against global cerebral ischemia. Proc Natl Acad Sci U S A 107:3204–3209

    Article  PubMed  CAS  Google Scholar 

  5. Mayr B, Montminy M (2001) Transcriptional regulation by the phosphorylation-dependent factor CREB. Nat Rev Mol Cell Biol 2: 599–609

    Article  PubMed  CAS  Google Scholar 

  6. Liu CQ, Shan L, Balesar R, Luchetti S, Van Heerikhuize JJ, Luo JH, Swaab DF, Bao AM (2010) A quantitative in situ hybridization protocol for formalin-fixed paraffin-embedded archival post-mortem human brain tissue. Methods 52(4):359–366

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Geriatric Research, Educational and Clinical Center, Veterans Affairs Pittsburgh Health Care System, Pittsburgh, PA, USA

    R. Anne Stetler

  2. Center of Cerebrovascular Disease Research, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    R. Anne Stetler

  3. State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, China

    R. Anne Stetler

  4. Department of Neurology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    Yu Gan

Authors
  1. R. Anne Stetler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yu Gan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. Anne Stetler .

Editor information

Editors and Affiliations

  1. School of Medicine, University of Pittsburgh, Terrace St. 3500, Pittsburgh, 15213, Pennsylvania, USA

    Jun Chen

  2. School of Medicine, Stark Neurosciences Res. Inst., Indiana University, W. Walnut St. 950, Indianapolis, 46202, Indiana, USA

    Xiao-Ming Xu

  3. School of Medicine, Department of Anatomy & Cell Biology, Indiana University, Barnhill Dr. 635, Indianapolis, 46202, Indiana, USA

    Zao C. Xu

  4. School of Medicine, Dept. Neurosurgery, Loma Linda University, Anderson St. 11234, Loma Linda, 92354, California, USA

    John H. Zhang

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Stetler, R.A., Gan, Y. (2012). Biochemical and Molecular Biological Assessments of Global Cerebral Ischemia: mRNA. In: Chen, J., Xu, XM., Xu, Z., Zhang, J. (eds) Animal Models of Acute Neurological Injuries II. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-61779-782-8_5

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-782-8_5

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-781-1

  • Online ISBN: 978-1-61779-782-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation