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Assessments of Gliogenesis After Spinal Cord Injury

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Animal Models of Acute Neurological Injuries II

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Spinal cord injury (SCI) results in a large proliferative response that serves to restore homeostatis and replenish cellular deficits. Postinjury repair and recovery can be interrogated as a function of the cell fates adopted by progenitors within the lesion. Administration of bromodeoxyuridine (BrdU) (by ip injection) and/or a recombinant retrovirus (by intraspinal injection) is used routinely to label progenitors in an injured spinal cord. In combination with immunofluorescence, confocal microscopy assesses the progeny of labeled-progenitors. Colocalization of phenotypic markers with BrdU or retroviral-reporters is used to determine the differentiation profile of progenitors to assess gliogenesis after SCI. This chapter introduces a brief history of labeling proliferative cells, required materials and tools to phenotype progeny, typical procedures, and variations.

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Author information

Authors and Affiliations

  1. Department of Neurological Surgery, Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA, USA

    Drew L. Sellers & Philip J. Horner

Authors
  1. Drew L. Sellers
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  2. Philip J. Horner
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Corresponding author

Correspondence to Drew L. Sellers .

Editor information

Editors and Affiliations

  1. School of Medicine, University of Pittsburgh, Terrace St. 3500, Pittsburgh, 15213, Pennsylvania, USA

    Jun Chen

  2. School of Medicine, Stark Neurosciences Res. Inst., Indiana University, W. Walnut St. 950, Indianapolis, 46202, Indiana, USA

    Xiao-Ming Xu

  3. School of Medicine, Department of Anatomy & Cell Biology, Indiana University, Barnhill Dr. 635, Indianapolis, 46202, Indiana, USA

    Zao C. Xu

  4. School of Medicine, Dept. Neurosurgery, Loma Linda University, Anderson St. 11234, Loma Linda, 92354, California, USA

    John H. Zhang

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Sellers, D.L., Horner, P.J. (2012). Assessments of Gliogenesis After Spinal Cord Injury. In: Chen, J., Xu, XM., Xu, Z., Zhang, J. (eds) Animal Models of Acute Neurological Injuries II. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-61779-782-8_40

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  • DOI: https://doi.org/10.1007/978-1-61779-782-8_40

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-781-1

  • Online ISBN: 978-1-61779-782-8

  • eBook Packages: Springer Protocols

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