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Random Amplified Marker Technique for Plants Rich in Polyphenols

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Plant DNA Fingerprinting and Barcoding

Part of the book series: Methods in Molecular Biology ((MIMB,volume 862))

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Abstract

More than 10,000 publications using the random amplified polymorphic DNA (RAPD) or related arbitrary marker techniques have been published in two decades of its inception in 1990. Despite extensive use, RAPD technique has also attracted some criticisms, mainly for lack of reproducibility. In the light of its widespread applications, the objective of this chapter is to (1) provide a protocol for RAPD assay, (2) identify the potential factors affecting the optimization of the RAPD assays, and (3) provide proper statistical analysis to avoid false positives. It is suggested that after proper optimization, the RAPD is a reliable, sensitive, and reproducible assay having the potential to detect a wide range of DNA variations. Analyses of the relevant fragments generated in RAPD profile allow not only to identify some of the molecular events implicated in the genomic instability but also to discover genes playing key roles in genetic evolution and gene mapping. RAPD markers will continue to be boon for genetic studies of those organisms where yet no sequence information or scanty information is available.

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Acknowledgments

We thankfully acknowledge the encouragement of Director, CIMAP and constant financial support by Council of Scientific and Industrial Research and Department of Biotechnology. The help of co-authors in our cited work is also acknowledged.

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Authors and Affiliations

  1. Department of Genetic Resource Management, Division of Genetics and Plant Breeding, Central Institute of Medicinal and Aromatic Plants, Lucknow, India

    Tripta Jhang

  2. Department of Plant Biotechnology, Division of Biotechnology, Central Institute of Medicinal and Aromatic Plants, Lucknow, India

    Ajit Kumar Shasany

Authors
  1. Tripta Jhang
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  2. Ajit Kumar Shasany
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Corresponding author

Correspondence to Ajit Kumar Shasany .

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Editors and Affiliations

  1. , Department of S.T.E.M., Roxbury Community College, Columbus Avenue 1234, Roxbury Crossing, 02120, Massachusetts, USA

    Nikolaus J. Sucher

  2. Centre for Complementary Medicine, Research, University of Western Sydney, Narellan Road, Campbelltown, 2560, New South Wales, Australia

    James R. Hennell

  3. , Department of Natural Sciences, Northern Essex Community College, Elliot Street 110, Haverhill, 01830, Massachusetts, USA

    Maria C. Carles

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Jhang, T., Shasany, A.K. (2012). Random Amplified Marker Technique for Plants Rich in Polyphenols. In: Sucher, N., Hennell, J., Carles, M. (eds) Plant DNA Fingerprinting and Barcoding. Methods in Molecular Biology, vol 862. Humana Press. https://doi.org/10.1007/978-1-61779-609-8_6

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  • DOI: https://doi.org/10.1007/978-1-61779-609-8_6

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-608-1

  • Online ISBN: 978-1-61779-609-8

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