Plant DNA Fingerprinting and Barcoding

Methods and Protocols

  • Nikolaus J. Sucher
  • James R. Hennell
  • Maria C. Carles

Part of the Methods in Molecular Biology book series (MIMB, volume 862)

Table of contents

  1. Front Matter
    Pages i-x
  2. Nikolaus J. Sucher, James R. Hennell, Maria C. Carles
    Pages 13-22
  3. Robyn S. Cowan, Michael F. Fay
    Pages 23-33
  4. David N. Zaya, Mary V. Ashley
    Pages 35-52
  5. Tripta Jhang, Ajit Kumar Shasany
    Pages 61-74
  6. Ok Ran Lee, Min-Kyeoung Kim, Deok-Chun Yang
    Pages 135-147
  7. Patricia L. Diaz, James R. Hennell, Nikolaus J. Sucher
    Pages 171-179
  8. James R. Hennell, Paul M. D’Agostino, Samiuela Lee, Cheang S. Khoo, Nikolaus J. Sucher
    Pages 181-200
  9. Back Matter
    Pages 201-202

About this book

Introduction

Molecular cloning and DNA-based analysis have become part of every molecular life science laboratory. The rapid adoption of DNA-based techniques has been facilitated by the introduction of the polymerase chain reaction (PCR), which has made cloning and characterization of DNA quick and relatively simple. PCR is virtually part of every variation of the plethora of approaches used for DNA fingerprinting today. Plant DNA Fingerprinting: Methods and Protocols aims to bring together the different currently available genome-based techniques into one repository. This volume contains detailed protocols for the preparation of plant genomic DNA, fingerprinting of plants for the detection of intra-species variations, the use of DNA barcoding, as well as methods for the bioinformatic analysis of data. Also included are several discussions on the broader issues of genome-based approaches in order to provide a sound understanding of the principles of these methods. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Plant DNA Fingerprinting: Methods and Protocols is tailored principally for those who seek to augment their current methods of plant analysis and quality control using genome-based approaches as well as for scientists and researchers in different plant sciences.

Keywords

DNA barcoding DNA sequencing plant genetics plant research plastid markers

Editors and affiliations

  • Nikolaus J. Sucher
    • 1
  • James R. Hennell
    • 2
  • Maria C. Carles
    • 3
  1. 1., Department of S.T.E.M.Roxbury Community CollegeRoxbury CrossingUSA
  2. 2.Centre for Complementary Medicine, ResearchUniversity of Western SydneyCampbelltownAustralia
  3. 3., Department of Natural SciencesNorthern Essex Community CollegeHaverhillUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-609-8
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-608-1
  • Online ISBN 978-1-61779-609-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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