Abstract
Transcript profiling is an invaluable strategy to study differential gene expression. Here we describe a detailed protocol for applying a subtractive hybridization technique, representational difference analysis (RDA), as a molecular strategy for the identification of differentially expressed genes in studies of host-fungus interaction. Bioinformatics tools that can be used in the analysis of expressed sequence tags (ESTs) are also detailed.
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Bailão, A.M., Pereira, M., Salem-Izacc, S.M., Borges, C.L., de Almeida Soares, C.M. (2012). Transcript Profiling Using ESTs from Paracoccidioides brasiliensis in Models of Infection. In: Brand, A., MacCallum, D. (eds) Host-Fungus Interactions. Methods in Molecular Biology, vol 845. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-61779-539-8_26
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DOI: https://doi.org/10.1007/978-1-61779-539-8_26
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Publisher Name: Humana, Totowa, NJ
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Online ISBN: 978-1-61779-539-8
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