Host-Fungus Interactions

Methods and Protocols

  • Alexandra C. Brand
  • Donna M. MacCallum

Part of the Methods in Molecular Biology book series (MIMB, volume 845)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Gene Disruption

  3. Modulation of Gene Expression: RNAi Gene Knockdown

    1. Front Matter
      Pages 149-149
    2. Michael L. Skowyra, Tamara L. Doering
      Pages 165-186
    3. João F. Menino, Agostinho J. Almeida, Fernando Rodrigues
      Pages 187-198
  4. Modulation of Gene Expression: Regulatable Promoters

    1. Front Matter
      Pages 199-199
    2. Michael Weyler, Joachim Morschhäuser
      Pages 201-210
    3. Lorina G. Baker, Jennifer K. Lodge
      Pages 211-226
    4. Vitor Cabral, Murielle Chauvel, Arnaud Firon, Mélanie Legrand, Audrey Nesseir, Sophie Bachellier-Bassi et al.
      Pages 227-244
  5. Host Responses to Infection In Vitro

    1. Front Matter
      Pages 245-245

About this book


Microbiologists, medical mycologists, immunologists, and biochemists are increasingly working together to focus on the processes involved in the progression and treatment of fungal disease.  Host-Fungus Interactions: Methods and Protocols is designed for research scientists who are involved in this work and interested in undertaking new or comparative studies of interactions between the mammalian host and clinically important fungal pathogens. Aiming to combine approaches for reverse genetics in pathogenic fungi with methods for their application in in vitro and in vivo models of disease, the book includes methods for the culture and genetic manipulation of the primary fungal pathogens and the opportunistic pathogens, as well as methods for investigating host-fungus interactions in model systems.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and practical, Host-Fungus Interactions: Methods and Protocols describes available molecular methods and fungal infection models in great detail in order to encourage researchers to try new approaches to investigating host-fungus interactions with added levels of confidence.


Fungal diseases Fungal infection models Genetic manipulation Host-fungus interactions Model systems Pathogenic fungi

Editors and affiliations

  • Alexandra C. Brand
    • 1
  • Donna M. MacCallum
    • 2
  1. 1.Institute of Medical Sciences, School of Medical SciencesUniversity of AberdeenAberdeenUnited Kingdom
  2. 2.Institute of Medical Sciences, School of Medical SciencesUniversity of AberdeenAberdeenUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-538-1
  • Online ISBN 978-1-61779-539-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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