Abstract
Planar cell polarity (PCP) is the polarization of cells within the plane of an epithelial cell layer. PCP is important in many tissues in different processes. In the kidney, it is hypothesized to be important in acquiring and maintaining correct tubular diameter. Aberrant PCP has been shown to be involved in polycystic kidney disease. Therefore, research in this field requires a method to study PCP. As PCP and outward-in signaling via the cilia are interconnected, the position of the centrosome, the base of the cilium can be used as a read-out system for PCP. Here, we provide a method in which the position of the centrosome is measured as read-out for PCP.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Fischer E, Legue E, Doyen A, Nato F, Nicolas JF, Torres V, Yaniv M, Pontoglio M (2006) Defective planar cell polarity in polycystic kidney disease. Nat Genet 38:21–23.
Patel V, Li L, Cobo-Stark P, Shao X, Somlo S, Lin F, Igarashi P (2008) Acute kidney injury and aberrant planar cell polarity induce cyst formation in mice lacking renal cilia. Hum Mol Genet 17:1578–1590.
Saburi S, Hester I, Fischer E, Pontoglio M, Eremina V, Gessler M, Quaggin SE, Harrison R, Mount R, McNeill H (2008) Loss of Fat4 disrupts PCP signaling and oriented cell division and leads to cystic kidney disease. Nat Genet 40:1010–1015.
Happe H, Leonhard WN, Van der Wal A, Van de Water B, Lantinga van Leeuwen IS, Breuning MH, De Heer E, Peters DJ (2009) Toxic tubular injury in kidneys from Pkd1-deletion mice accelerates cystogenesis accompanied by dysregulated planar cell polarity and canonical Wnt signaling pathways. Hum Mol Genet 18:2532–2542.
Jonassen JA, San Agustin J, Follit JA, Pazour GJ (2008) Deletion of IFT20 in the mouse kidney causes misorientation of the mitotic spindle and cystic kidney disease. J Cell Biol 183:377–384.
Acknowledgments
We thank A. van der Wal and F. Prins for technical assistance.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Happé, H., de Heer, E., Peters, D.J.M. (2012). Morphometric Analysis of Centrosome Position in Tissues. In: Turksen, K. (eds) Planar Cell Polarity. Methods in Molecular Biology, vol 839. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-510-7_20
Download citation
DOI: https://doi.org/10.1007/978-1-61779-510-7_20
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-61779-509-1
Online ISBN: 978-1-61779-510-7
eBook Packages: Springer Protocols