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Capillary Electrophoresis of Big-Dye Terminator Sequencing Reactions for Human mtDNA Control Region Haplotyping in the Identification of Human Remains

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DNA Electrophoresis Protocols for Forensic Genetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 830))

Abstract

Cycle sequencing reaction with Big-Dye terminators provides the methodology to analyze mtDNA Control Region amplicons by means of capillary electrophoresis. DNA sequencing with ddNTPs or terminators was developed by (1). The progressive automation of the method by combining the use of fluorescent-dye terminators with cycle sequencing has made it possible to increase the sensibility and efficiency of the method and hence has allowed its introduction into the forensic field. PCR-generated mitochondrial DNA products are the templates for sequencing reactions. Different set of primers can be used to generate amplicons with different sizes according to the quality and quantity of the DNA extract providing sequence data for different ranges inside the Control Region.

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Authors and Affiliations

  1. Comisaría General de Policía Científica, Servicio de Analítica, Laboratorio de ADN, Instituto Universitario de Investigación en Ciencias Policiales (IUICP), Madrid, Spain

    Marta Montesino & Lourdes Prieto

Authors
  1. Marta Montesino
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  2. Lourdes Prieto
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Correspondence to Marta Montesino .

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Editors and Affiliations

  1. Ciencias Forenses, Servicio de Biología, Instituto Nacional de Toxicología y, C/ José Echegaray 4, Las Rozas, 28232, Madrid, Spain

    Antonio Alonso

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Montesino, M., Prieto, L. (2012). Capillary Electrophoresis of Big-Dye Terminator Sequencing Reactions for Human mtDNA Control Region Haplotyping in the Identification of Human Remains. In: Alonso, A. (eds) DNA Electrophoresis Protocols for Forensic Genetics. Methods in Molecular Biology, vol 830. Humana Press. https://doi.org/10.1007/978-1-61779-461-2_19

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  • DOI: https://doi.org/10.1007/978-1-61779-461-2_19

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-460-5

  • Online ISBN: 978-1-61779-461-2

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