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DNA Electrophoresis Protocols for Forensic Genetics

  • Antonio Alonso

Part of the Methods in Molecular Biology book series (MIMB, volume 830)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Current Protocols on Capillary Electrophoresis of Amplified DNA Fragments

    1. Front Matter
      Pages 1-1
    2. Robyn Thompson, Silvia Zoppis, Bruce McCord
      Pages 3-16
    3. Leonor Gusmão, Cíntia Alves, Iva Gomes, Paula Sánchez-Diz
      Pages 57-71
    4. C. Phillips, M. Fondevila, Maria Victoria Lareau
      Pages 109-126
    5. René Huel, Sylvain Amory, Ana Bilić, Stojko Vidović, Edin Jasaragić, Thomas J. Parsons
      Pages 185-198
  3. Capillary Electrophoresis and DNA Sequencing

  4. Advances in Microchip Capillary Electrophoresis

  5. Back Matter
    Pages 391-394

About this book

Introduction

Forensic DNA profiling procedures are mainly based on high resolution and high throughput capillary electrophoresis separation and detection systems of  PCR amplicons obtained from DNA genomic markers with different  inheritance patterns. In DNA Electrophoresis Protocols for Forensic Genetics, expert researchers in the field detail many of the protocols and methods which are now commonly used to perform forensic DNA profiling. It  includes protocols for  profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and  mtDNA  hypervariable regions HV1 and HV2 . Protocols for molecular identification of non-human species and mRNA profiling for body fluid identification are also included. Written in the highly successful  Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Keywords

DNA STR markers autosomal STRs capillary electrophoresis forensic botany forensic genetics forensic veterinary microbial forensic molecular identification sex chromosome markers

Editors and affiliations

  • Antonio Alonso
    • 1
  1. 1.Ciencias Forenses, Servicio de BiologíaInstituto Nacional de Toxicología yLas RozasSpain

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-461-2
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-460-5
  • Online ISBN 978-1-61779-461-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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