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Capillary Electrophoresis of an X-Chromosome STR Decaplex for Kinship Deficiency Cases

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DNA Electrophoresis Protocols for Forensic Genetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 830))

Abstract

During the two last decades, STR markers located on the autosomes have been gaining relevance and have nearly replaced the use of other type of markers in most cases of genetic identification, paternity testing, as well as in other situations of kinship analysis. Nevertheless, in some complex cases, independently of the number of polymorphisms being typed, autosomal markers convey very little information. Depending on the parentage constellation available for analysis, as well as the gender of the subjects, this problem can sometimes be solved by using markers that have different modes of transmission. Therefore, most forensic laboratories are nowadays prepared to analyse lineage markers (Y chromosome and mtDNA) and many have recently set up methods for the analysis of X-STRs. In the present chapter, a method is described for the typing of ten X chromosome-specific markers in a single PCR amplification reaction, followed by capillary electrophoresis separation and fluorescent detection in an ABI Genetic Analyser apparatus. This typing strategy was developed and optimized for the simultaneous amplification of ten X-linked specific STRs well distributed along the chromosome: DXS8378, DXS9902, DXS7132, DXS9898, DXS6809, DXS6789, DXS7133, GATA172D05, GATA31E08 and DXS7423.

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Acknowledgments

IPATIMUP is an Associate Laboratory of the Portuguese Ministry of Science, Technology and Higher Education and is partially supported by FCT, the Portuguese Foundation for Science and Technology.

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Authors and Affiliations

  1. IPATIMUP—Institute of Pathology and Molecular Immunology of the University of Porto, Porto, Portugal

    Leonor Gusmão, Cíntia Alves & Iva Gomes

  2. Genetic Unit, Department of Pathologic Anatomy and Forensic Sciences, Institute of Legal Medicine, University of Santiago de Compostela, Galicia, Spain

    Paula Sánchez-Diz

Authors
  1. Leonor Gusmão
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  2. Cíntia Alves
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  3. Iva Gomes
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  4. Paula Sánchez-Diz
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Corresponding author

Correspondence to Leonor Gusmão .

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Editors and Affiliations

  1. Ciencias Forenses, Servicio de Biología, Instituto Nacional de Toxicología y, C/ José Echegaray 4, Las Rozas, 28232, Madrid, Spain

    Antonio Alonso

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Gusmão, L., Alves, C., Gomes, I., Sánchez-Diz, P. (2012). Capillary Electrophoresis of an X-Chromosome STR Decaplex for Kinship Deficiency Cases. In: Alonso, A. (eds) DNA Electrophoresis Protocols for Forensic Genetics. Methods in Molecular Biology, vol 830. Humana Press. https://doi.org/10.1007/978-1-61779-461-2_5

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  • DOI: https://doi.org/10.1007/978-1-61779-461-2_5

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-460-5

  • Online ISBN: 978-1-61779-461-2

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