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Determination of MiRNA Targets in Skeletal Muscle Cells

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Myogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 798))

Abstract

MicroRNAs (miRNAs) are a class of small ∼22 nucleotide noncoding RNAs which regulate gene expression at the posttranscriptional level by either destabilizing and consequently degrading their targeted mRNAs or by repressing their translation. Emerging evidence has demonstrated that miRNAs are essential for normal mammalian development, homeostasis, and many other functions. In addition, deleterious changes in miRNA expression were associated with human diseases. Several muscle-specific miRNAs, including miR-1, miR-133, miR-206, and miR-208, have been shown to be important for normal myoblast differentiation, proliferation, and muscle remodeling in response to stress. They have also been implicated in various cardiac and skeletal muscular diseases. miRNA-based gene therapies hold great potential for the treatment of cardiac and skeletal muscle diseases. Herein, we describe methods commonly applied to study the biological role of miRNAs, as well as techniques utilized to manipulate miRNA expression and to investigate their target regulation.

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Acknowledgments

We thank members of the Wang laboratory for discussion and ­support. Research in the Wang lab was supported by the March of Dimes Birth Defect Foundation, National Institutes of Health and Muscular Dystrophy Association. ZP Huang is a postdoctoral ­fellow and DZ Wang is an Established Investigator of the American Heart Association.

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Authors and Affiliations

  1. Cardiovascular Research Division, Department of Cardiology, Children’s Hospital Boston, Harvard Medical School, Boston, MA, USA

    Zhan-Peng Huang, Ramón Espinoza-Lewis & Da-Zhi Wang

Authors
  1. Zhan-Peng Huang
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  2. Ramón Espinoza-Lewis
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  3. Da-Zhi Wang
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Corresponding author

Correspondence to Da-Zhi Wang .

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Editors and Affiliations

  1. Chicago Medical School, Dept. Cell Biology & Anatomy, Rosalind Franklin Univ of Med & Sci, Green Bay Rd. 3333, North Chicago, 60064, Illinois, USA

    Joseph X. DiMario

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Huang, ZP., Espinoza-Lewis, R., Wang, DZ. (2012). Determination of MiRNA Targets in Skeletal Muscle Cells. In: DiMario, J. (eds) Myogenesis. Methods in Molecular Biology, vol 798. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-343-1_28

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  • DOI: https://doi.org/10.1007/978-1-61779-343-1_28

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-342-4

  • Online ISBN: 978-1-61779-343-1

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