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Myogenesis

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Joseph X. DiMario

    1. Chicago Medical School, Dept. Cell Biology & Anatomy, Rosalind Franklin Univ of Med & Sci, North Chicago, USA

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  • Presents model systems and state-of-the-art techniques developed and perfected by leading scientists in the field
  • Includes step-by-step protocols that are ready for the lab
  • Features expert tips and key implementation advice in order to ensure successful results

Part of the book series: Methods in Molecular Biology (MIMB, volume 798)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xv
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  2. Isolation and Analysis of Skeletal Muscle Precursor Cells

    1. Front Matter

      Pages 1-1
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    2. Isolation and Characterization of Human Fetal Myoblasts

      • Ariya D. Lapan, Emanuela Gussoni
      Pages 3-19

    3. Skeletal Muscle Satellite Cells: Background and Methods for Isolation and Analysis in a Primary Culture System

      • Maria Elena Danoviz, Zipora Yablonka-Reuveni
      Pages 21-52

    4. Isolation of Muscle Stem Cells by Fluorescence Activated Cell Sorting Cytometry

      • Alessandra Pasut, Paul Oleynik, Michael A. Rudnicki
      Pages 53-64

    5. Mouse and Human Mesoangioblasts: Isolation and Characterization from Adult Skeletal Muscles

      • Mattia Quattrocelli, Giacomo Palazzolo, Ilaria Perini, Stefania Crippa, Marco Cassano, Maurilio Sampaolesi
      Pages 65-76

    6. Direct Electrical Stimulation of Myogenic Cultures for Analysis of Muscle Fiber Type Control

      • Eric J. Cavanaugh, Jennifer R. Crew, Joseph X. DiMario
      Pages 77-84

    7. Single Muscle-Fiber Isolation and Culture for Cellular, Molecular, Pharmacological, and Evolutionary Studies

      • Judy E. Anderson, Ashley C. Wozniak, Wataru Mizunoya
      Pages 85-102

    8. Somite Unit Chronometry to Analyze Teratogen Phase Specificity in the Paraxial Mesoderm

      • Sara J. Venters, Charles P. Ordahl
      Pages 103-123

  3. Non-Mammalian Models of Myogenesis

    1. Front Matter

      Pages 125-125
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    2. Analysis of Skeletal Muscle Development in Drosophila

      • Ginny R. Morriss, Anton L. Bryantsev, Maria Chechenova, Elisa M. LaBeau, TyAnna L. Lovato, Kathryn M. Ryan et al.
      Pages 127-152

    3. Immunocytochemistry to Study Myogenesis in Zebrafish

      • Nathan C. Bird, Stefanie E. Windner, Stephen H. Devoto
      Pages 153-169

    4. Immunofluorescent Localization of Proteins in Caenorhabditis elegans Muscle

      • Kristy J. Wilson, Hiroshi Qadota, Guy M. Benian
      Pages 171-181

  4. Experimental Models and Analysis of Skeletal Muscle Exercise and Disuse

    1. Front Matter

      Pages 183-183
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    2. Resistance Loading and Signaling Assays for Oxidative Stress in Rodent Skeletal Muscle

      • Stephen E. Alway, Robert G. Cutlip
      Pages 185-211

    3. Analysis of Skeletal Muscle Hypertrophy in Models of Increased Loading

      • Sue C. Bodine, Keith Baar
      Pages 213-229

    4. Protein Overexpression in Skeletal Muscle Using Plasmid-Based Gene Transfer to Elucidate Mechanisms Controlling Fiber Size

      • Chia-Ling Wu, Susan C. Kandarian
      Pages 231-243

    5. In Vivo Measurement of Muscle Protein Synthesis Rate Using the Flooding Dose Technique

      • Marta L. Fiorotto, Horacio A. Sosa Jr., Teresa A. Davis
      Pages 245-264

  5. Generation of Viral Vectors and Transgenic Mice

    1. Front Matter

      Pages 265-265
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    2. Recombinant Adeno-Associated Viral Vector Production and Purification

      • Jin-Hong Shin, Yongping Yue, Dongsheng Duan
      Pages 267-284
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Keywords

About this book

Our understanding of the molecular and cellular mechanisms that control skeletal muscle development, regeneration, and adaptive responses to activity has increased dramatically in recent years, fostered by innovative techniques and approaches that are either specifically designed or adapted for research in skeletal muscle biology.  Myogenesis: Methods and Protocols presents detailed, step-by-step methods in the study of the molecular and cellular biology of skeletal muscle cells.  Protocols from different model systems including mammalian, avian, zebrafish, and invertebrate skeletal muscle are included in this volume.  Highlighted topics cover a wide range of interests and expertise including myogenic and stem cell isolation, investigation of models of exercise and disuse, viral vector delivery systems, calcium imaging, cell profiling, as well as protein-DNA and protein-protein interactions. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and authoritative, Myogenesis: Methods and Protocols serves as an invaluable, state-of-the-art resource for experienced and emerging scientists in basic research as well as clinical and regenerative medicine.

Editors and Affiliations

  • Chicago Medical School, Dept. Cell Biology & Anatomy, Rosalind Franklin Univ of Med & Sci, North Chicago, USA

    Joseph X. DiMario

Bibliographic Information

  • Book Title: Myogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Joseph X. DiMario

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-343-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-342-4Published: 01 November 2011

  • Softcover ISBN: 978-1-4939-5890-0Published: 23 August 2016

  • eBook ISBN: 978-1-61779-343-1Published: 02 December 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 558

  • Number of Illustrations: 71 b/w illustrations, 15 illustrations in colour

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

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