Abstract
Increased vascular permeability is the hallmark of inflammation. Here, we describe three methods to assess vascular permeability in cell culture: (1) Impedance measurements of endothelial cell monolayers that allow to monitor changes in cell shape in real time. (2) Diffusion of fluorescently labeled dextran across endothelial cell monolayers to identify openings large enough for bulky molecules. (3) Transmigration of neutrophils through confluent endothelial cell monolayers to study one major process that increases endothelial permeability in inflammation.
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Acknowledgments
This work was supported by the IZKF Aachen and by the DFG, SFB 542, Projects A12 and C16.
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Ludwig, A., Sommer, A., Uhlig, S. (2011). Assessment of Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cell Monolayers. In: Turksen, K. (eds) Permeability Barrier. Methods in Molecular Biology, vol 763. Humana Press. https://doi.org/10.1007/978-1-61779-191-8_22
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DOI: https://doi.org/10.1007/978-1-61779-191-8_22
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