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  • © 2011

Permeability Barrier

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols covering a variety of permeability barrier
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and vital implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 763)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xv
  2. Computational Modeling of the Skin Barrier

    • Arne Naegel, Michael Heisig, Gabriel Wittum
    Pages 1-32
  3. In Vitro Human Skin Segmentation and Drug Concentration–Skin Depth Profiles

    • Ana Melero, Tsambika Hahn, Ulrich F. Schaefer, Marc Schneider
    Pages 33-50
  4. Transcriptional Regulation of Epidermal Barrier Formation

    • Ambica Bhandari, Michael L. Salmans, William Gordon, Bogi Andersen
    Pages 51-71
  5. Epidermal Permeability Barrier Measurement in Mammalian Skin

    • Arup Kumar Indra, Mark Leid
    Pages 73-81
  6. Assessment of Intestinal Permeability in (Premature) Neonates by Sugar Absorption Tests

    • Willemijn E. Corpeleijn, Ruurd M. van Elburg, Ido P. Kema, Johannes B. van Goudoever
    Pages 95-104
  7. Analysis of Epithelial Cell Shedding and Gaps in the Intestinal Epithelium

    • Carrie A. Duckworth, Alastair J. Watson
    Pages 105-114
  8. Studying Permeability in a Commonly Used Epithelial Cell Line: T84 Intestinal Epithelial Cells

    • Rino P. Donato, Adaweyah El-Merhibi, Batjargal Gundsambuu, Kai Yan Mak, Emma R. Formosa, Xian Wang et al.
    Pages 115-137
  9. Ouabain Modulates Cell Contacts as well as Functions that Depend on Cell Adhesion

    • Isabel Larre, Ruben G. Contreras, Marcelino Cereijido
    Pages 155-168
  10. Measuring Permeability in Human Retinal Epithelial Cells (ARPE-19): Implications for the Study of Diabetic Retinopathy

    • Marta Garcia-Ramírez, Marta Villarroel, Lídia Corraliza, Cristina Hernández, Rafael Simó
    Pages 179-194
  11. Permeability of Differentiated Human Urothelium In Vitro

    • Peter Rubenwolf, Jennifer Southgate
    Pages 207-222
  12. Phenotyping the Claudin 11 Deficiency in Testis: From Histology to Immunohistochemistry

    • Séverine Mazaud-Guittot, Alexander Gow, Brigitte Le Magueresse-Battistoni
    Pages 223-236
  13. Analysis of Endothelial Barrier Function In Vitro

    • Yuping Wang, J. Steven Alexander
    Pages 253-264
  14. In Vitro Analyses of Endothelial Cell Permeability

    • Elizabeth Monaghan-Benson, Erika S. Wittchen
    Pages 281-290

About this book

The significant biological subject, the permeability barrier, is incredibly diverse and vital for a vast assortment of crucial functions in the body.  In Permeability Barrier: Methods and Protocols, a variety of experienced researchers contribute techniques to study this complex system in its many forms.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Permeability Barrier: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research.

Editors and Affiliations

  • Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Permeability Barrier

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-191-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-190-1Published: 31 August 2011

  • Softcover ISBN: 978-1-4939-5862-7Published: 04 May 2017

  • eBook ISBN: 978-1-61779-191-8Published: 28 August 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 450

  • Number of Illustrations: 79 b/w illustrations, 22 illustrations in colour

  • Topics: Cell Biology, Cell Physiology

  • Industry Sectors: Biotechnology, Pharma

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access