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Ouabain Modulates Cell Contacts as well as Functions that Depend on Cell Adhesion

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Permeability Barrier

Part of the book series: Methods in Molecular Biology ((MIMB,volume 763))

Abstract

Ouabain, a toxic of vegetal origin used for centuries to treat heart failure, has recently been demonstrated to have an endogenous counterpart, most probably ouabain itself, which behaves as a hormone. Therefore, the challenge now is to discover the physiological role of hormone ouabain. We have recently shown that it modulates cell contacts such as gap junctions, which communicate neighboring cells, as well as tight junctions (TJs), which are one of the two differentiated features of epithelial cells, the other being apical/basolateral polarity. The importance of cell contacts can be hardly overestimated, since the most complex object in the universe, the brain, assembles itself depending on what cells contacts what other(s) how, when, and how is the molecular composition and special arrangement of the contacts involved. In the present chapter, we detail the protocols used to demonstrate the effect of ouabain on the molecular structure and functional properties of one of those cell–cell contacts: the TJ.

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Authors and Affiliations

  1. Center for Research & Advanced Studies (CINVESTAV), Mexico City, México

    Isabel Larre & Ruben G. Contreras

  2. Department of Physiology, Biophysics and Neurosciences, Center for Research & Advanced Studies (CINVESTAV), Av. Instituto Politécnico Nacional 2508, Col. San Pedro Zacatenco, Del. GAM C.P., 07360, México, México

    Marcelino Cereijido

Authors
  1. Isabel Larre
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  2. Ruben G. Contreras
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  3. Marcelino Cereijido
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Corresponding author

Correspondence to Marcelino Cereijido .

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Editors and Affiliations

  1. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Smyth Road 501, Ottawa, K1Y 8L6, Canada

    Kursad Turksen

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Larre, I., Contreras, R.G., Cereijido, M. (2011). Ouabain Modulates Cell Contacts as well as Functions that Depend on Cell Adhesion. In: Turksen, K. (eds) Permeability Barrier. Methods in Molecular Biology, vol 763. Humana Press. https://doi.org/10.1007/978-1-61779-191-8_10

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  • DOI: https://doi.org/10.1007/978-1-61779-191-8_10

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-190-1

  • Online ISBN: 978-1-61779-191-8

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