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Ex Vivo Expansion of Haematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation

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Cell Cycle Synchronization

Part of the book series: Methods in Molecular Biology ((MIMB,volume 761))

Abstract

The efficient use of haematopoietic stem cells (HSC) for transplantation is often limited by the relatively low numbers of HSC collected. The ex vivo expansion of HSC for clinical use is a potentially valuable and safe approach to increase HSC numbers thereby increasing engraftment and reducing the risk of morbidity from infection. Here we describe a protocol for the robust ex vivo expansion of human CD34(+) HSC isolated from umbilical cord blood. The protocol described can efficiently generate large numbers of HSC. We also describe a flow cytometry-based method using high resolution division tracking to characterise the kinetics of HSC growth and differentiation. Utilising the guidelines discussed, it is possible for investigators to use this protocol as presented or to modify it for their specific needs.

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Acknowledgements

The authors thank the Australian Cord Blood Bank for providing cord blood.

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Authors and Affiliations

  1. Sydney Cord & Marrow Transplant Facility, Graduate School of Biomedical Engineering, Sydney Children’s Hospital, University of New South Wales, Kensington, NSW, Australia

    Kap-Hyoun Ko

  2. Graduate School of Biomedical Engineering, University of New South Wales, Kensington, NSW, Australia

    Robert Nordon

  3. Sydney Cord & Marrow Transplant Facility, Centre for Children’s Cancer & Blood Disorders, Sydney Children’s Hospital, Sydney, Australia

    Tracey A. O’Brien

  4. Faculty of Medicine, University of New South Wales, Kensington, NSW, Australia

    Geoff Symonds

  5. Sydney Cord & Marrow Transplant Facility, Faculty of Medicine, Sydney Children’s Hospital, University of New South Wales, Sydney, NSW, 2031, Australia

    Alla Dolnikov

Authors
  1. Kap-Hyoun Ko
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  2. Robert Nordon
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  3. Tracey A. O’Brien
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  4. Geoff Symonds
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  5. Alla Dolnikov
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Corresponding author

Correspondence to Alla Dolnikov .

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Editors and Affiliations

  1. , Dept. Microbial Biotech. & Cell Biology, University of Debrecen, Egyetem Square 1, Debrecen, 4010, Hungary

    Gaspar Banfalvi

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Ko, KH., Nordon, R., O’Brien, T.A., Symonds, G., Dolnikov, A. (2011). Ex Vivo Expansion of Haematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation. In: Banfalvi, G. (eds) Cell Cycle Synchronization. Methods in Molecular Biology, vol 761. Humana Press. https://doi.org/10.1007/978-1-61779-182-6_17

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  • DOI: https://doi.org/10.1007/978-1-61779-182-6_17

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-181-9

  • Online ISBN: 978-1-61779-182-6

  • eBook Packages: Springer Protocols

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