Cell Cycle Synchronization

Methods and Protocols

  • Gaspar Banfalvi

Part of the Methods in Molecular Biology book series (MIMB, volume 761)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Gaspar Banfalvi
    Pages 1-23
  3. Maria Castedo, Lorenzo Galluzzi, Ilio Vitale, Laura Senovilla, Didier Métivier, Mohamed Jèmaà et al.
    Pages 47-63
  4. Kalyan Dulla, Anna Santamaria
    Pages 65-74
  5. Thomas J. Langan, Richard C. Chou
    Pages 75-83
  6. Jennifer A. Fischer, Salvatore Caradonna
    Pages 137-149
  7. Hoi Tang Ma, Randy Y.C. Poon
    Pages 151-161
  8. Arkadi Manukyan, Lesley Abraham, Huzefa Dungrawala, Brandt L. Schneider
    Pages 173-200
  9. Staffan Svärd, Karin Troell
    Pages 201-210
  10. Tamas Somfai, Yuji Hirao
    Pages 211-225
  11. Ferhan Ayaydin, Edit Kotogány, Edit Ábrahám, Gábor V. Horváth
    Pages 227-238
  12. Kap-Hyoun Ko, Robert Nordon, Tracey A. O’Brien, Geoff Symonds, Alla Dolnikov
    Pages 249-260
  13. Fabienne Venet, Caroline Guignant, Guillaume Monneret
    Pages 261-275
  14. Attila Csikász-Nagy, Alida Palmisano, Judit Zámborszky
    Pages 277-291

About this book

Introduction

To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe.  In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events.  Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the “arrest-and-release” approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Detailed and easy to follow,  Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so.

Keywords

Bacteria Mammalian cells Model systems Plant cells Regulatory mechanisms Stability Storage conditions Synchronized cells Toxicity of reagents Yeast and fungal cells

Editors and affiliations

  • Gaspar Banfalvi
    • 1
  1. 1., Dept. Microbial Biotech. & Cell BiologyUniversity of DebrecenDebrecenHungary

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-182-6
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-181-9
  • Online ISBN 978-1-61779-182-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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