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Micropropagation of Phalaenopsis Orchids via Protocorms and Protocorm-Like Bodies

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Plant Embryo Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 710))

Abstract

Phalaenopsis orchids have high economic value in the floriculture industry. Hybridization or cross-pollination in the breeding program have proven to be very reliable techniques for the production of a wide range of successful cultivars with attractive combinations of spray length, bud number, flower color and type, fragrance, seasonality, and compactness. In vitro propagation makes it possible to clonally mass propagate hybrids of commercial value and conserved species. However, in vitro culture technologies are still a challenge because of the slow growth of plantlets, low multiplication rate, poor rooting, and somaclonal variation. Although seed-raised plants can be used for conservation and breeding for the selection of superior features, genetic characteristics including seasonality, inflorescence, flower color, and type are not uniform. In this regard, micropropagation through protocorm-like bodies obtained from germinating embryos and somatic tissues is an important strategy in obtaining genetically stable plants and the improvement of quality. However, not all genotypes of Phalaenopsis respond to the same protocol under the same culture conditions and often result in the development of undesirable characteristics. In this chapter, plantlet production in Phalaenopsis orchids via the culture of protocorms from seeds and protocorm-like bodies from leaf sections and root tips are detailed.

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Author information

Authors and Affiliations

  1. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheong-ju, Republic of Korea

    Kee Yoeup Paek & Eun Joo Hahn

  2. Biotechnology Division, Korea Forest Research Institute, Suwon, Republic of Korea

    So Young Park

Authors
  1. Kee Yoeup Paek
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  2. Eun Joo Hahn
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  3. So Young Park
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Editor information

Editors and Affiliations

  1. Dept. Biological Sciences, University of Calgary, University Dr. NW., 2500, Calgary, T2N 1N4, Alberta, Canada

    Trevor A. Thorpe

  2. , Department of Biological Sciences, University of Calgary, University Dr. NW 2500, Calgary, T2N 1N4, Alberta, Canada

    Edward C. Yeung

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© 2011 Humana Press

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Paek, K.Y., Hahn, E.J., Park, S.Y. (2011). Micropropagation of Phalaenopsis Orchids via Protocorms and Protocorm-Like Bodies. In: Thorpe, T., Yeung, E. (eds) Plant Embryo Culture. Methods in Molecular Biology, vol 710. Humana Press. https://doi.org/10.1007/978-1-61737-988-8_20

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  • DOI: https://doi.org/10.1007/978-1-61737-988-8_20

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61737-987-1

  • Online ISBN: 978-1-61737-988-8

  • eBook Packages: Springer Protocols

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