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Plant Embryo Culture

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Trevor A. Thorpe

    1. Dept. Biological Sciences, University of Calgary, Calgary, Canada

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    You can also search for this editor in PubMed   Google Scholar

  2. Edward C. Yeung

    1. , Department of Biological Sciences, University of Calgary, Calgary, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Presents detailed zygotic embryo isolation techniques with a wide variety of applications
  • Serves as a quick, convenient reference source
  • Includes tips from the labs of experts, well-versed in the methods and protocols provided

Part of the book series: Methods in Molecular Biology (MIMB, volume 710)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
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  2. Culture of Zygotic Embryos: Fundamental Studies

    1. Front Matter

      Pages 1-1
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    2. Zygotic Embryo Culture: An Overview

      • Tegan M. Haslam, Edward C. Yeung
      Pages 3-15

    3. In Vitro Fertilization with Rice Gametes: Production of Zygotes and Zygote and Embryo Culture

      • Takashi Okamoto
      Pages 17-27

    4. Canola Zygotic Embryo Culture

      • Nicole S. Ramesar-Fortner, Edward C. Yeung
      Pages 29-37

    5. Immature Seeds and Embryos of Medicago truncatula Cultured In Vitro

      • Sergio J. Ochatt
      Pages 39-52

    6. In Vitro Culture and Germination of Terrestrial Asian Orchid Seeds

      • Yung-I. Lee
      Pages 53-62

    7. In Vitro Culture of Coconut (Cocos nucifera L.) Zygotic Embryos

      • Florent Engelmann, Bernard Malaurie, Oulo N’Nan
      Pages 63-72

  3. Application of Embryo Culture Techniques: Embryo Rescue

    1. Front Matter

      Pages 73-73
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    2. Immature Embryo Rescue and Culture

      • Xiuli Shen, Fred G. Gmitter Jr., Jude W. Grosser
      Pages 75-92

    3. Chickpea Hybridization Using In Vitro Techniques

      • Nalini Mallikarjuna, Fred J. Muehlbauer
      Pages 93-105

    4. Muskmelon Embryo Rescue Techniques Using In Vitro Embryo Culture

      • Hector Gordon Nuñez-Palenius, Rafael Ramírez-Malagón, Neftalí Ochoa-Alejo
      Pages 107-115

    5. Phaseolus Immature Embryo Rescue Technology

      • Pascal Geerts, André Toussaint, Guy Mergeai, Jean-Pierre Baudoin
      Pages 117-129

    6. Wide Crossing in Lentil through Embryo Rescue

      • Richard Fratini, Maria L. Ruiz
      Pages 131-139

    7. Generation of Interspecific Hybrids of Trifolium Using Embryo Rescue Techniques

      • Ajoy Kumar Roy, Devendra Ram Malaviya, Pankaj Kaushal
      Pages 141-151

  4. Cryopreservation of Embryos

    1. Front Matter

      Pages 153-153
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    2. Cryopreservation of Embryos: An Overview

      • Florent Engelmann
      Pages 155-184

    3. Cryogenic Technologies for the Long-Term Storage of Citrus Germplasm

      • Anna De Carlo, Maurizio Lambardi, Elif Aylin Ozudogru
      Pages 185-200

    4. Cryopreservation of Zygotic Embryonic Axes and Somatic Embryos of European Chestnut

      • Ana M. Vieitez, M. Carmen San-José, Elena Corredoira
      Pages 201-213

    5. Cryopreservation of Ilex Immature Zygotic Embryos

      • Luis Mroginski, Natalia Dolce, Pedro Sansberro, Claudia Luna, Ana Gonzalez, Hebe Rey
      Pages 215-225
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Keywords

About this book

A great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant embryology promises to lead to the development of numerous useful applications. In Plant Embryo Culture: Methods and Protocols, expert researchers from the field provide a ready source of information for culturing zygotic embryos for different types of studies, both theoretical and practical. The book’s main sections examine a wide range of related topics, including the culture of zygotic embryos for developmental studies, the application of embryo culture techniques focusing on embryo rescue methods, cryopreservation of zygotic embryos, the use of zygotic embryos as explants for somatic embryogenesis and organogenesis, as well as transformation protocols using zygotic embryos as starting material. Written in the highly successful Methods in Molecular Biology™ series format, the detailed chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital notes on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Plant Embryo Culture: Methods and Protocols serves as a key reference that can be used by scientists of all backgrounds to help develop their own customized methods for many different species and for a variety of purposes.

Editors and Affiliations

  • Dept. Biological Sciences, University of Calgary, Calgary, Canada

    Trevor A. Thorpe

  • , Department of Biological Sciences, University of Calgary, Calgary, Canada

    Edward C. Yeung

Bibliographic Information

  • Book Title: Plant Embryo Culture

  • Book Subtitle: Methods and Protocols

  • Editors: Trevor A. Thorpe, Edward C. Yeung

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61737-988-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61737-987-1Published: 11 January 2011

  • Softcover ISBN: 978-1-4939-5789-7Published: 23 August 2016

  • eBook ISBN: 978-1-61737-988-8Published: 05 January 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 377

  • Topics: Plant Sciences, Cell Culture

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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