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Clinical Proteomics: Liquid Chromatography–Mass Spectrometry Purification Systems

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Protein Chromatography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 681))

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Abstract

Liquid chromatography/mass spectrometry (LC/MS) has become a routine powerful technology in clinical proteomic studies in protein identification, protein characterisation, and the discovery of biomarkers. In this chapter, we describe simple protocols for protein digestion and the analysis of digested peptides produced both by one-dimensional reversed-phase liquid chromatography and two-dimensional liquid chromatography (i.e. strong cation exchange chromatography followed by reversed-phase chromatography), coupled with tandem mass spectrometry.

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Author information

Authors and Affiliations

  1. National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland

    Michael Henry & Paula Meleady

Authors
  1. Michael Henry
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  2. Paula Meleady
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Corresponding author

Correspondence to Paula Meleady .

Editor information

Editors and Affiliations

  1. National Centre for Sensor Research, School of Biotechnology, Dublin City University, Dublin, 9, Ireland

    Dermot Walls

  2. National Centre for Sensor Research, School of Biotechnology, Dublin City University, Dublin, 9, Ireland

    Sinéad T. Loughran

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© 2011 Springer Science+Business Media, LLC

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Henry, M., Meleady, P. (2011). Clinical Proteomics: Liquid Chromatography–Mass Spectrometry Purification Systems. In: Walls, D., Loughran, S. (eds) Protein Chromatography. Methods in Molecular Biology, vol 681. Humana Press. https://doi.org/10.1007/978-1-60761-913-0_28

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  • DOI: https://doi.org/10.1007/978-1-60761-913-0_28

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-912-3

  • Online ISBN: 978-1-60761-913-0

  • eBook Packages: Springer Protocols

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