Protein Chromatography

Methods and Protocols

  • Dermot Walls
  • Sinéad T. Loughran

Part of the Methods in Molecular Biology book series (MIMB, volume 681)

Table of contents

  1. Front Matter
    Pages i-x
  2. Overviews

    1. Front Matter
      Pages 1-1
    2. Dermot Walls, Robert McGrath, Sinéad T. Loughran
      Pages 3-23
    3. Ciarán Ó’Fágáin, Philip M. Cummins, Brendan F. O’Connor
      Pages 25-33
    4. Jenny Fitzgerald, Paul Leonard, Elaine Darcy, Richard O’Kennedy
      Pages 35-59
    5. Ciarán Ó’Fágáin
      Pages 103-136
    6. Frank A. Gomez
      Pages 137-150
    7. Dermot Walls, Sinéad T. Loughran
      Pages 151-175
  3. Protocols

    1. Front Matter
      Pages 177-177
    2. Ciarán Ó’Fágáin
      Pages 179-202
    3. Eva M. Campion, Sinéad T. Loughran, Dermot Walls
      Pages 229-258
    4. Sandra Harper, David W. Speicher
      Pages 259-280
    5. Mario Lebendiker, Tsafi Danieli
      Pages 281-293
    6. Luke O’Shaughnessy, Sean Doyle
      Pages 295-309
    7. Sinéad T. Loughran, Dermot Walls
      Pages 311-335

About this book

Introduction

A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.

Keywords

Chromatographic methods Microfluidics Native protein isolation Protein biochemistry Protein quantitation Purification schemes Recombinant antibody production

Editors and affiliations

  • Dermot Walls
    • 1
  • Sinéad T. Loughran
    • 2
  1. 1.National Centre for Sensor Research, School of BiotechnologyDublin City UniversityDublinIreland
  2. 2.National Centre for Sensor Research, School of BiotechnologyDublin City UniversityDublinIreland

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-913-0
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-912-3
  • Online ISBN 978-1-60761-913-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book

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