Abstract
Expression of recombinant proteins in the baculovirus/insect cell expression system is employed because it enables post-translational protein modification and high yields of recombinant protein. The system is capable of facilitating the functional expression of many proteins – either secreted or intracellularly located within infected insect cells. Strategies for the isolation and extraction of soluble proteins are presented in this chapter and involve selective cell lysis, precipitation and chromatography. Protein insolubility, following recombinant expression in insect cells, can occur. However, using the methods described herein, it is possible to extract and purify insoluble protein using affinity, ion-exchange and gel filtration chromatography. Indeed, protein insolubility often aids protein purification.
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Acknowledgments
Luke O’Shaughnessy is supported by a Strategic Research Cluster Grant (BioNanoInteract; 07/SRC/B1155) from Science Foundation Ireland.
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O’Shaughnessy, L., Doyle, S. (2011). Purification of Proteins from Baculovirus-Infected Insect Cells. In: Walls, D., Loughran, S. (eds) Protein Chromatography. Methods in Molecular Biology, vol 681. Humana Press. https://doi.org/10.1007/978-1-60761-913-0_16
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DOI: https://doi.org/10.1007/978-1-60761-913-0_16
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