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Pre-embedding Immunoelectron Microscopy of Chemically Fixed Mammalian Tissue Culture Cells

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Immunoelectron Microscopy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 657))

Abstract

Immunoelectron microscopy is one of the best methods for detecting and localizing protein molecules in cells and tissues. Gold particles of 1.4 nm in diameter (Nanogold) conjugated with Fab’ fragments easily penetrate into the cell interior and are used for pre-embedding immunoelectron microscopy. To obtain a contrast for the gold label, silver enhancement of the gold particles is essential. By changing the intensity of the silver enhancement, the size of the granules can be controlled. In this chapter, we described the use of Nanogold for pre-embedding immunoelectron microscopy of paraformaldehyde-fixed cultured cells.

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Acknowledgments

This study was supported, in part, by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, & Technology of Japan.

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Authors and Affiliations

  1. Department of Occupational Therapy, School of Health Sciences, Faculty of Medicine, Gunma University, Gunma, Japan

    Haruo Hagiwara

  2. Department of Anatomy and Cell Biology, Gunma University Graduate School of Medicine, Gunma, Japan

    Takeo Aoki, Takeshi Suzuki & Kuniaki Takata

Authors
  1. Haruo Hagiwara
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  2. Takeo Aoki
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  3. Takeshi Suzuki
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  4. Kuniaki Takata
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Editor information

Editors and Affiliations

  1. Dept. Biology, University of Memphis, Walker Avenue 3774, Memphis, 38152, Tennessee, USA

    Steven D. Schwartzbach

  2. Dept. Life Science, Nippon Sport Science University, Kamosida 1221-1, Yokohama, 227-0033, Japan

    Tetsuaki Osafune

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Hagiwara, H., Aoki, T., Suzuki, T., Takata, K. (2010). Pre-embedding Immunoelectron Microscopy of Chemically Fixed Mammalian Tissue Culture Cells. In: Schwartzbach, S., Osafune, T. (eds) Immunoelectron Microscopy. Methods in Molecular Biology, vol 657. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-783-9_11

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  • DOI: https://doi.org/10.1007/978-1-60761-783-9_11

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-782-2

  • Online ISBN: 978-1-60761-783-9

  • eBook Packages: Springer Protocols

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