Immunoelectron Microscopy

Methods and Protocols

  • Steven D. Schwartzbach
  • Tetsuaki Osafune

Part of the Methods in Molecular Biology book series (MIMB, volume 657)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Molecular Toolbox

    1. Front Matter
      Pages 1-1
    2. David M. Rancour, Steven K. Backues, Sebastian Y. Bednarek
      Pages 3-20
    3. Melanie L. Styers, Jason Lowery, Elizabeth Sztul
      Pages 43-61
    4. Masami Nakazawa, Mari Mukumoto, Kazutaka Miyatake
      Pages 63-74
    5. Masami Nakazawa, Mari Mukumoto, Kazutaka Miyatake
      Pages 75-91
    6. Bao-Shiang Lee, Jin-Sheng Huang, G. D. Lasanthi P. Jayathilaka, Syed S. Lateef, Shalini Gupta
      Pages 93-108
  3. Microscopy Toolbox

    1. Front Matter
      Pages 121-121
    2. Tetsuaki Osafune, Steven D. Schwartzbach
      Pages 123-132
    3. Haruo Hagiwara, Takeo Aoki, Takeshi Suzuki, Kuniaki Takata
      Pages 145-154
    4. Miyuki Takeuchi, Keiji Takabe, Yoshinobu Mineyuki
      Pages 155-165
    5. Shinichi Ohno, Nobuhiko Ohno, Nobuo Terada, Sei Saitoh, Yurika Saitoh, Yasuhisa Fujii
      Pages 167-179
    6. Jindriska Fiserova, Martin W. Goldberg
      Pages 191-204

About this book

Introduction

Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Immunoelectron Microscopy: Methods and Protocols seeks to facilitate an increased understanding of structure function relationships.

Keywords

Antigen Fixation protocols Macromolecular functions Monoclonal Antibodies Organelle Pre- and post-embedding immunogold labeling Structure function relationships Vivo biochemistry electron microscopy escherichia coli genes molecular biology rDNA saccharomyces cerevisiae

Editors and affiliations

  • Steven D. Schwartzbach
    • 1
  • Tetsuaki Osafune
    • 2
  1. 1.Dept. BiologyUniversity of MemphisMemphisUSA
  2. 2.Dept. Life ScienceNippon Sport Science UniversityYokohamaJapan

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-783-9
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-782-2
  • Online ISBN 978-1-60761-783-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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