Abstract
Spider venom contains a complex mixture of components with a large range of molecular masses (0.1–60 kDa) exhibiting a diverse array of actions. Most of these components are proteinaceous molecules – biologically active proteins and peptides. Proteomics profiling of spider venoms (the components with MW >10 kDa) could be achieved through conventional 2-DE-based proteomics methods combined with MS or MS/MS detection. Peptidomic profiling (of the components with MW below ~10 kDa) is usually achieved through off-line separation by a combination of ion-exchange and reverse-phase chromatography, and it relies more heavily on de novo sequencing by Edman degradation or MS/MS for peptide identification.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Escoubas, P., Sollod, B., and King, G.F. (2006) Venom landscapes: mining the complexity of spider venoms via a combined cDNA and mass spectrometric approach. Toxicon 47, 650–663.
Escoubas, P. (2006) Mass spectrometry in toxicology: a 21st-century technology for the study of biopolymers from venoms. Toxicon 47, 609–613.
Yuan, C., Jin, Q., Tang, X., Hu, W., Cao, R., Yang, S., Xiong, J., Xie, C., Xie, J., and Liang, S. (2007) Proteomic and peptidomic characterization of the venom from the Chinese bird spider, Ornithoctonus huwena Wang. J. Proteome Res. 6, 2792–2801.
Liao, Z., Cao, J., Li, S., Yan, X., Hu, W., He, Q., Chen, J., Tang, J., Xie, J., and Liang, S. (2007) Proteomic and peptidomic analysis of the venom from Chinese tarantula Chilobrachys jingzhao. Proteomics 7, 1892–1907.
Huang, L., Li, B., Luo, C., Xie, J., Chen, P., and Liang, S. (2004) Proteome comparative analysis of gynogenetic haploid and diploid embryos of goldfish (Carassius auratus). Proteomics 4, 235–243.
Reinders, J., Lewandrowski, U., Moebius, J., Wagner, Y., and Sickmann, A. (2004) Challenges in mass spectrometry-based proteomics. Proteomics 4, 3686–3703.
Glish, G.L. and Burinsky, D.J. (2008) Hybrid mass spectrometers for tandem mass spectrometry. J. Am. Soc. Mass Spectrom. 19, 161–172.
Shevchenko, A., Sunyaev, S., Loboda, A., Shevchenko, A., Bork, P., Ens, W., and Standing, K.G. (2001) Charting the proteomes of organisms with unsequenced genomes by MALDI-quadrupole time-of-flight mass spectrometry and BLAST homology searching. Anal. Chem. 73, 1917–1926.
Escoubas, P., Quinton, L., and Nicholson, G.M. (2008) Venomics: unravelling the complexity of animal venoms with mass spectrometry. J. Mass Spectrom. 43, 279–295.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Songping, L. (2010). Protocols for Peptidomic Analysis of Spider Venoms. In: Soloviev, M. (eds) Peptidomics. Methods in Molecular Biology, vol 615. Humana Press. https://doi.org/10.1007/978-1-60761-535-4_6
Download citation
DOI: https://doi.org/10.1007/978-1-60761-535-4_6
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-60761-534-7
Online ISBN: 978-1-60761-535-4
eBook Packages: Springer Protocols