Peptidomics

Methods and Protocols

  • Mikhail Soloviev

Part of the Methods in Molecular Biology book series (MIMB, volume 615)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Mikhail Soloviev
      Pages 3-9
  3. From Bacteria to Men

    1. Front Matter
      Pages 11-11
    2. Joshua N. Adkins, Heather Mottaz, Thomas O. Metz, Charles Ansong, Nathan P. Manes, Richard D. Smith et al.
      Pages 13-27
    3. Steven J. Husson, Elke Clynen, Kurt Boonen, Tom Janssen, Marleen Lindemans, Geert Baggerman et al.
      Pages 29-47
    4. Ka Wan Li, August B. Smit
      Pages 49-56
    5. Alexander A. Vassilevski, Sergey A. Kozlov, Tsezi A. Egorov, Eugene V. Grishin
      Pages 87-100
    6. Christian Wegener, Susanne Neupert, Reinhard Predel
      Pages 117-127
    7. Joachim Schachtner, Christian Wegener, Susanne Neupert, Reinhard Predel
      Pages 129-135
    8. Susanne Neupert, Reinhard Predel
      Pages 137-144
    9. J. Michael Conlon, Jérôme Leprince
      Pages 145-157
    10. Kurt Boonen, Steven J. Husson, Bart Landuyt, Geert Baggerman, Eisuke Hayakawa, Walter H.M.L. Luyten et al.
      Pages 191-206

About this book

Introduction

Despite being known and studied for years, peptides have never before attracted enough attention to necessitate the invention of the term "peptidomics" in order to specify the study of the complement of peptides from a cell, organelle, tissue or organism. In Peptidomics: Methods and Protocols, expert researchers present a comprehensive range of analytical techniques for the analysis of the peptide contents of complex biological samples with an emphasis often on higher throughput techniques, suitable for the analysis of large numbers of peptides typically present in the peptidomes. Encompassing a number of species ranging from bacteria to man, the methods presented intensively cover topics such as organism handling, tissue and organ dissection, cellular and subcellular fractionation, peptide extraction, fractionation and purification, structural characterization, molecular cloning, and sequence analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Peptidomics: Methods and Protocols brings this ten year old field fully up-to-date in order to inspire novices and experts alike with the easy-to-follow practical advice needed to set up and carry out analysis of the peptide contents of complex biological samples.

Keywords

DNA Hydra Microarray Proteomics Vivo biosynthesis cell lines molecular biology

Editors and affiliations

  • Mikhail Soloviev
    • 1
  1. 1.School of Biological SciencesRoyal Holloway University of LondonEgham, SurreyUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-535-4
  • Copyright Information Humana Press, a part of Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-534-7
  • Online ISBN 978-1-60761-535-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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