Abstract
Our understanding of human lymphocyte development has increased significantly over the past 20 years. In particular, our insight into human T- and B-cell development has improved (1, 2). Nonetheless, there are many gaps in our understanding, particularly regarding the early stages of development of hematopoietic progenitor cells (HPCs) into downstream lineage-biased and lineage-restricted precursors and the molecular mechanisms underlying these activities. The same holds true for our knowledge of human dendritic cell (DC) development. While the amount of data on the different subsets of conventional DCs (cDCs) and plasmacytoid DCs (pDCs) rapidly increases in mice (3, 4), the developmental stages of different DC subsets in humans remain poorly defined (2). The relatively easy access to patient material and therefore human precursor cells that can be isolated from these tissues combined with the availability of in vitro and in vivo differentiation assays allows studies in the field of human hematopoietic development, including that of DCs. In addition, the opportunities to manipulate gene expression, by stable overexpression of a gene of interest or RNA interference-mediated knockdown, generate valuable information about the mechanisms underlying lineage commitment and differentiation.
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Acknowledgments
We thank previous members of the Spits-lab for their contribution in optimizing the procedures described here. Berend Hooijbrink is acknowledged for his help with FACS sorting and maintenance of the FACS facility. Dr M. Hazekamp and staff at the Leiden University Medical Center and the Amsterdam Medical Center are acknowledged for generously providing postnatal thymus tissue. We are grateful to the Bloemenhove Clinic (Heemstede, the Netherlands) and the Department of Gynecology of the AMC for providing fetal tissues and cord blood, respectively. OP9 cells were provided by T. Nakano (Osaka University, Osaka, Japan). Our work is supported by a ZonMW-VIDI fellowship, NIH grant R01 (AI52002), Dutch Cancer Foundation (KWF), Landsteiner Blood Transfusion Research Foundation (LSBR), and the Bill and Melinda Gates Foundation through the Grand Challenges in Global Health program (Human Vaccine Consortium).
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Schotte, R. et al. (2010). Isolation and In Vitro Generation of Gene-Manipulated Human Plasmacytoid and Conventional Dendritic Cells. In: Naik, S. (eds) Dendritic Cell Protocols. Methods in Molecular Biology, vol 595. Humana Press. https://doi.org/10.1007/978-1-60761-421-0_5
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DOI: https://doi.org/10.1007/978-1-60761-421-0_5
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