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Dendritic Cell Protocols

  • Shalin H. Naik

Part of the Methods in Molecular Biology book series (MIMB, volume 595)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Part I: Reviews

    1. Front Matter
      Pages 1-1
    2. Xinsheng Ju, Georgina Clark, Derek N.J. Hart
      Pages 3-20
    3. Tewfik Miloud, Günter J. Hämmerling, Natalio Garbi
      Pages 21-42
  3. Part II: Human DC Protocols

    1. Front Matter
      Pages 43-43
    2. Andrew J. Kassianos, Sarah L. Jongbloed, Derek N.J. Hart, Kristen J. Radford
      Pages 45-54
    3. Marein A.W.P. de Jong, Lot de Witte, Teunis B.H. Geijtenbeek
      Pages 55-65
    4. Remko Schotte, Heike Schmidlin, Maho Nagasawa, Wendy Dontje, Julien J. Karrich, Christel Uittenbogaart et al.
      Pages 67-85
    5. Anja U. van Lent, Mireille Centlivre, Maho Nagasawa, Julien J. Karrich, Stephan M. Pouw, Kees Weijer et al.
      Pages 87-115
    6. Pawel Kalinski, Eva Wieckowski, Ravikumar Muthuswamy, Esther de Jong
      Pages 117-133
    7. Hth R. Turnquist, Ryan T. Fischer, Angus W. Thomson
      Pages 135-148
  4. Part III: Murine Dendritic Cell Methods

    1. Front Matter
      Pages 165-165
    2. Shalin H. Naik, Meredith O’Keeffe, Anna Proietto, Hubertus Hochrein Ken Shortman, Li Wu
      Pages 167-176
    3. Meredith O’Keeffe, Ben Fancke, Hubertus Hochrein
      Pages 187-193
    4. Nobuyuki Onai, Markus G. Manz, Michael A. Schmid
      Pages 195-203
    5. Julie Helft, Miriam Merad
      Pages 231-233
    6. Patrizia Stoitzner, Nikolaus Romani, Alexander D. McLellan, Christoph H. Tripp, Susanne Ebner
      Pages 235-248
    7. Philip A. Stumbles, Deborah H. Strickland, Mathew E. Wikstrom, Jennifer A. Thomas, Christophe von Garnier, Patrick G. Holt
      Pages 249-263
    8. Li Wu, Angela D’Amico
      Pages 265-280
    9. Simon Milling, Gordon MacPherson
      Pages 281-297
    10. Adele M. Mount, Gabrielle T. Belz
      Pages 299-318
    11. Michael Neuenhahn, Matthias Schiemann, Dirk H. Busch
      Pages 319-329
    12. Philippe Pouliot, Monique A. Willart, Hamida Hammad, Bart N. Lambrecht
      Pages 331-349
    13. Claudia Jakubzick, Gwendalyn J. Randolph
      Pages 371-382
    14. Ariel Savina, Pablo Vargas, Pierre Guermonprez, Ana-Maria Lennon, Sebastian Amigorena
      Pages 383-402
  5. Back Matter
    Pages 443-446

About this book

Introduction

Given the vital importance of immune system research, the gathering of clear, consistent, and informative protocols involving the study of dendritic cells is paramount. Bringing the popular first edition fully up to date, Dendritic Cell Protocols, Second Edition presents protocols from experts in the field that cover the basics and more complex forays into the exploration of DC development and function, both in mice and humans. The first section of the volume involving humans explores topics such as the isolation of blood DC subtypes, primary skin Langerhans cells, and the generation of gene-manipulated human DCs with the inclusion of more clinically relevant methods as well, while the second section involving rodent models delves into DC and precursor generation in vitro, isolation ex vivo, disease models, as well as DC functions and properties. Written in the highly successful Methods in Molecular Biology™ series style, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Dendritic Cell Protocols, Second Edition aims to become a bench-side handbook for both beginners and experts in the field of DC research and a long-term reference for some of the most popular methods put forward by those who lead the field.

Keywords

Activation Antigen Asthma Immune System Laboratory Migration Transplantation Vivo gene expression genes molecular biology

Editors and affiliations

  • Shalin H. Naik
    • 1
  1. 1.AmsterdamNetherlands

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-421-0
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-420-3
  • Online ISBN 978-1-60761-421-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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