Abstract
In the steady-state lymphoid organ, dendritic cells (DCs) are classified into two major subsets, plasmacytoid DC (pDC) and conventional DC (cDC). A standing question was whether a common progenitor for plasmacytoid and conventional dendritic cells exists during the sequential differentiation from hematopoietic stem cells to dendritic cells. We have recently identified such a common clonogenic plasmacytoid and dendritic cell progenitor (CDP) from mouse bone marrow using antibodies for c-kit, Flt3, and M-CSFR. CDPs generated almost exclusively pDC and cDC in vitro and upon transfer in irradiated and steady-state mice in vivo. Single-cell analysis revealed the existence of clonal progenitors giving rise to both pDC and cDC within the CDP population. Thus, these results prove the existence of a common developmental pathway for at least some pDCs and cDCs in lymphoid organs in vivo.
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Acknowledgments
Supported in part by the Deutsche Forschungsgemeinschaft (MA 2159/2-1), the Swiss National Science Foundation (310000-116637), and the European Commission FP6 “Network of Excellence” initiative under contract number LSHB-CT-2004-512074 DC-THERA.
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Onai, N., Manz, M.G., Schmid, M.A. (2010). Isolation of Common Dendritic Cell Progenitors (CDP) from Mouse Bone Marrow. In: Naik, S. (eds) Dendritic Cell Protocols. Methods in Molecular Biology, vol 595. Humana Press. https://doi.org/10.1007/978-1-60761-421-0_13
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DOI: https://doi.org/10.1007/978-1-60761-421-0_13
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