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Electron Microscopy of Mammalian Chromosomes

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Meiosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 558))

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Abstract

This chapter describes methods of preparation of meiotic nuclei for their analysis with electron microscopy. Procedures include surface spreading as well as fixation, staining, and sectioning of tissue and subsequent 3-D reconstruction analysis. Immunostaining protocols for electron microscopy are provided. The rationale for using electron microscopy in place of light or fluorescent microscopy for specific cytological analyses is presented.

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Author information

Authors and Affiliations

  1. Department of Biology, York University, Toronto, Ontario, Canada

    Peter Moens

Authors
  1. Peter Moens
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Editor information

Editors and Affiliations

  1. Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, York Ave. 1275, New York, 10021-6007, U.S.A.

    Scott Keeney

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Moens, P. (2009). Electron Microscopy of Mammalian Chromosomes. In: Keeney, S. (eds) Meiosis. Methods in Molecular Biology, vol 558. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-103-5_19

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  • DOI: https://doi.org/10.1007/978-1-60761-103-5_19

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-102-8

  • Online ISBN: 978-1-60761-103-5

  • eBook Packages: Springer Protocols

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