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Validation of Bioluminescent Imaging Techniques

  • John Virostko
  • E. Duco Jansen
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 574)

Abstract

Bioluminescence imaging (BLI) is frequently cited for its ease of quantification. This fundamental strength of BLI has led to applications in cancer research, cell transplantation, and monitoring of infectious disease in which bioluminescence intensity is correlated with other metrics. However, bioluminescence measurements can be influenced by a number of factors, among them source location, tissue optical properties, and substrate availability and pharmacokinetics. Accounting for these many factors is crucial for accurate BLI quantification. A number of methods can be employed to ensure correct interpretation of BLI results and validate BLI techniques. This chapter summarizes the use of calibrated light-emitting standards, bioluminescence tomography, and post-mortem validation of luciferase expression for validating quantitative BLI measurements.

Key words

Bioluminescence imaging BLI luciferase optical imaging quantification validation optical attenuation light propagation bioluminescence tomography 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • John Virostko
    • 1
  • E. Duco Jansen
    • 2
  1. 1.Vanderbilt University Institute of Imaging Science, Vanderbilt UniversityNashvilleUSA
  2. 2.Department of Biomedical EngineeringVanderbilt UniversityNashvilleUSA

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