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Validation of Bioluminescent Imaging Techniques

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Bioluminescence

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 574))

Abstract

Bioluminescence imaging (BLI) is frequently cited for its ease of quantification. This fundamental strength of BLI has led to applications in cancer research, cell transplantation, and monitoring of infectious disease in which bioluminescence intensity is correlated with other metrics. However, bioluminescence measurements can be influenced by a number of factors, among them source location, tissue optical properties, and substrate availability and pharmacokinetics. Accounting for these many factors is crucial for accurate BLI quantification. A number of methods can be employed to ensure correct interpretation of BLI results and validate BLI techniques. This chapter summarizes the use of calibrated light-emitting standards, bioluminescence tomography, and post-mortem validation of luciferase expression for validating quantitative BLI measurements.

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Author information

Authors and Affiliations

  1. Vanderbilt University Institute of Imaging Science, Vanderbilt University, Nashville, TN, USA

    John Virostko

  2. Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA

    E. Duco Jansen

Authors
  1. John Virostko
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  2. E. Duco Jansen
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Editor information

Editors and Affiliations

  1. Dept. Surgery, University of North Carolina, Chapel Hill, 27599-7228, U.S.A.

    Preston B. Rich

  2. Dept. Surgery, University of North Carolina, Manning Drive 90, Chapel Hill, 27599-7161, U.S.A.

    Christelle Douillet

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Virostko, J., Jansen, E.D. (2009). Validation of Bioluminescent Imaging Techniques. In: Rich, P., Douillet, C. (eds) Bioluminescence. Methods in Molecular Biology™, vol 574. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-321-3_2

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  • DOI: https://doi.org/10.1007/978-1-60327-321-3_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-320-6

  • Online ISBN: 978-1-60327-321-3

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