Editors:
- Provides an easily accessible reference volume for a wide range of bioluminescence assays and applications written by leading researchers in the field
- Comprehensive guide to developing, validating, running, and analyzing bioluminescence assays
- Develops detection and imaging methods
- Describes the use of a wide variety of bioluminescence tools: various luciferases, reporter gene systems including PIN-G reporters, light emitting bacteria, smart probes, BRET, quantums dots, transgenic animals, etc.
- Presents protocols with enough details to be followed and adapted by scientific teams with no previous experience in bioluminescence
- Contains numerous notes about potential pitfalls and tips on how to avoid failures
- Presents cutting-edge material that will also interest experts in the field of bioluminescence
- Provides methods and assays that can be adapted and used in most specialties of biomedical and biotechnological fields: studies of cell trafficking, protein-protein interactions, intracellular signaling, apoptosis, RNAi knockdown, bacterial or viral infections, transplanted cells, stem cells proliferation, vascular flow, and tumors
- Covers a wide range of sub-cellular and cell-based assays for in vitro and in vivo analysis as well as non invasive, in vivo, and real time assays and monitoring for animal models
- Also includes methods for high throughput analysis of animal models
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 574)
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Table of contents (20 protocols)
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Front Matter
About this book
Reviews
Editors and Affiliations
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Dept. Surgery, University of North Carolina, Chapel Hill, U.S.A.
Preston B. Rich, Christelle Douillet
Bibliographic Information
Book Title: Bioluminescence
Book Subtitle: Methods and Protocols
Editors: Preston B. Rich, Christelle Douillet
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-321-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-60327-320-6Published: 31 August 2009
Softcover ISBN: 978-1-61779-752-1Published: 07 March 2012
eBook ISBN: 978-1-60327-321-3Published: 15 August 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XII, 268
Number of Illustrations: 31 b/w illustrations, 29 illustrations in colour
Topics: Biochemistry, general, Biological and Medical Physics, Biophysics, Biochemical Engineering
Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma