Summary
A method for the construction of West Nile virus immune donor antibody repertoires is described. B cells are harvested from a suitable donor and the antibody variable genes are amplified using polymerase chain reaction (PCR). The PCR fragments are cloned in a phage display vector to construct a repertoire that can be used in panning procedures to identify many unique monoclonal antibodies.
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References
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Throsby, M., de Kruif, J. (2009). Construction of Phage Antibody Repertoires from the Blood of West Nile Virus-Infected Donors. In: Aitken, R. (eds) Antibody Phage Display. Methods in Molecular Biology, vol 562. Humana Press. https://doi.org/10.1007/978-1-60327-302-2_4
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DOI: https://doi.org/10.1007/978-1-60327-302-2_4
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Online ISBN: 978-1-60327-302-2
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