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Antibody Phage Display

Methods and Protocols

  • Robert Aitken

Part of the Methods in Molecular Biology book series (MIMB, volume 562)

Table of contents

  1. Front Matter
    Pages i-x
  2. Javier Cabiedes, Antonio R. Cabral
    Pages 61-70
  3. Iain Shaw, Marian Kane
    Pages 83-97
  4. Mitchell Ho, Ira Pastan
    Pages 99-113
  5. Bao Guo-qiang, He Xian-Li
    Pages 133-142
  6. Sergey M. Kipriyanov
    Pages 177-193
  7. Laurence Guglielmi, Pierre Martineau
    Pages 195-203
  8. Laurence Guglielmi, Pierre Martineau
    Pages 215-224
  9. Leonardo M. Damasceno, Frank Lee, Gerd Ritter, Lloyd Old, Carl Batt
    Pages 225-236
  10. Back Matter
    Pages 237-240

About this book

Introduction

Since its introduction almost 20 years ago, phage display technology has revolutionized approaches to the analysis of biomedical problems, quickly impacting the fields of  immunology, cell biology, biotechnology, pharmacology, and drug discovery. In Antibody Phage Display: Methods and Protocols, Second Edition, expert researchers explore the latest in this cutting-edge technology, providing an invaluable resource that will guide readers in the design and execution of experiments based around antibody phage display. Chapters present a wide range of methods of isolating recombinant antibodies from phage display libraries, examine how the targets recognized by antibodies of interest can be identified, discuss the identification and exploitation of antibodies that can enter cells and bind to cytosolic targets, and include novel approaches to the expression of recombinant antibodies. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Detailed and innovative, Antibody Phage Display: Methods and Protocols, Second Edition is a critical handbook on phage display technology which is certain to stimulate the reader’s imagination as much as it will guide future practice in the laboratory.

Keywords

Antigen Filamentous phage Phage display library Recombinant antibodies Target antigen cytoplasm molecular biology virus

Editors and affiliations

  • Robert Aitken

There are no affiliations available

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-302-2
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-301-5
  • Online ISBN 978-1-60327-302-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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