Summary
The broad application of recombinant adenoviruses to the development of vaccines and gene therapy vectors has encouraged the development of molecular assays for the facile quantitation of adenoviral particles and the assignment of their infectious potency. The Genome Quantitation Assay (GQA) and the QPCR-Based Potency Assay (QPA) developed for adenoviruses offer the attributes of precision, rapidity, and high throughput either performed manually or facilitated by simple automated liquid handling systems. These assay attributes allow for accelerated process development support and product characterization and release. The assays for adenovirus could offer the additional advantage in that their quantitation is based on viral replication independent of cytopathology permitting quantitation of serotypes that cause minimal cytopathic effect (CPE) in 293 cells and specificity that allows the components of multivalent vaccines to be discriminated and quantitated for release.
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Acknowledgments
The authors thank our colleagues who provided strong support throughout this work (alphabetically): Andrew Bett, Barry Buckland, Carrie Harper, Anthonise Louis, Jennifer McMackin, Allen Puddy, Volker Sandig, Timothy Schofield, Robert Sitrin, Charles Tan, Jenny Xu, and Yuhua Zhang.
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Wang, F., Mathis, B.C., Montalvo, A., Wolf, J.J., Lewis, J.A. (2008). Quantifying the Titer and Quality of Adenovirus Stocks. In: Le Doux, J.M. (eds) Gene Therapy Protocols. Methods in Molecular Biology™, vol 434. Humana Press. https://doi.org/10.1007/978-1-60327-248-3_3
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DOI: https://doi.org/10.1007/978-1-60327-248-3_3
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