Overview
- Collects time tested, reproducible laboratory protocols, including tricks and hints, written by experts in the field
- Covers current and emerging methods for production of viral and non-viral gene transfer vectors
- Provides approaches to design vectors for targeted and regularted gene delivery and expression
Part of the book series: Methods in Molecular Biology (MIMB, volume 434)
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Table of contents (22 protocols)
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Front Matter
Keywords
About this book
In Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today’s technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this second volume of Gene Therapy Protocols: Design and Characterization of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the processing and characterization of viral and non-viral gene transfer vectors, as well as promising approaches to design vectors for efficient, targeted and regulated gene delivery and expression. This second volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.
Reviews
From the reviews of the third edition:
"This book reflects progress in the field with chapters on early stage vector development and in vitro evaluation, as well as others devoted to issues faced in preclinical and clinical development … . Readers of this book are likely to be those working in vector development, validation and production as well as those involved in gene-based clinical trials. The specialized nature of this book means that it will primarily be used by experienced researchers … ." (Christopher Ring, Microbiology Today, June, 2009)
Editors and Affiliations
Bibliographic Information
Book Title: Gene Therapy Protocols
Book Subtitle: Volume 2: Design and Characterization of Gene Transfer Vectors
Editors: Joseph M. Doux
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-248-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-60327-247-6Published: 24 April 2008
Softcover ISBN: 978-1-62703-964-2Published: 04 November 2014
eBook ISBN: 978-1-60327-248-3Published: 10 May 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XII, 313
Topics: Human Genetics, Animal Genetics and Genomics, Gene Therapy
Industry Sectors: Biotechnology, Pharma