Summary
Adenovirus vectors currently are being evaluated in gene delivery studies ranging from prophylactic vaccination to therapeutic gene therapy. The quantity of purified virus required for these studies necessitate that purification methods must shift from classical density gradient ultracentrifugation to scaleable approaches. A methodology is described herein using batch centrifugation, tangential flow ultrafiltration, and chromatography to purify adenovirus particles at a scale of approximately \(10^{13}\) viral particles. This method has been demonstrated to easily scale an additional 40-fold. While purification of human adenovirus type 5 is exemplified, modifications are suggested for the purification of other serotypes.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Konz, J.O., Pitts, L.R., Sagar, S.L. (2008). Scaleable Purification of Adenovirus Vectors. In: Le Doux, J.M. (eds) Gene Therapy Protocols. Methods in Molecular Biology™, vol 434. Humana Press. https://doi.org/10.1007/978-1-60327-248-3_2
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DOI: https://doi.org/10.1007/978-1-60327-248-3_2
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Online ISBN: 978-1-60327-248-3
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