Summary
A comprehensive understanding of regulatory protein interactions with their target genes is fundamental to determining transcriptional networks and identifying important events in the regulation of gene expression. Here we describe how transcriptional regulatory regions are to be identified using luciferase assays (including the transfection of cells by Amaxa and lipid-based reagents) and how protein—DNA interactions are to be characterised by chromatin immunoprecipitation (ChIP) coupled with quantitative PCR. Together these techniques provide a powerful combination for investigating potassium channel gene regulation.
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Acknowledgments
This work was supported by the British Heart Foundation and the Wellcome Trust.
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Ooi, L., Wood, I.C. (2008). Identifying Transcriptional Regulatory Regions Using Reporter Genes and DNA—Protein Interactions by Chromatin Immunoprecipitation. In: Lippiat, J.D. (eds) Potassium Channels. Methods in Molecular Biology, vol 491. Humana Press. https://doi.org/10.1007/978-1-59745-526-8_1
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DOI: https://doi.org/10.1007/978-1-59745-526-8_1
Publisher Name: Humana Press
Print ISBN: 978-1-934115-65-7
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