Potassium Channels

Methods and Protocols

  • Jonathan D. Lippiat

Part of the Methods in Molecular Biology book series (MIMB, volume 491)

Table of contents

  1. Front Matter
    Pages i-x
  2. Methods For Studying Potassium Channel Transcription And Mrna Splicing

  3. Methods for Studying the Cell Biology of Potassium Channel Proteins

  4. Electrophysiological Techniques For The Study Of Potassium Channel Function

    1. Andrea Brüggemann, Cecilia Farre, Claudia Haarmann, Ali Haythornthwaite, Mohamed Kreir, Sonja Stoelzle et al.
      Pages 165-176
    2. Heidi de Wet, Jonathan D. Lippiat, Marcus Allen
      Pages 177-186
  5. Optical Techniques For The Study Of Potassium Channel Function And Interactions

  6. Methods For Studying Potassium Channelopathies And Pharmacological Modulators

    1. Sarah E. Flanagan, Sian Ellard
      Pages 235-245
    2. Mark L. Dallas, Susan A. Deuchars, Jim Deuchars
      Pages 247-255

About this book

Introduction

Due to the importance of potassium channels in cellular function, researchers have been challenged to develop tools and experimental procedures to probe the function and identify chemicals that modulate the behaviour of this super-family of ion channels. In Potassium Channels: Methods and Protocols, experts in the field present a range of experimental approaches that have been developed to investigate potassium channel structure, function, pharmacology, cell biology, gene expression, and their role in disease. Many of these techniques study potassium channels as cellular proteins as well as the resultant membrane biophysics. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section, which highlights tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Potassium Channels: Methods and Protocols provides a wide range of approaches in order to aid current research projects and inspire new investigations.

Keywords

Cellular function Cellular proteins Electrophysiological techniques Ion channels Membrane excitability Optical techniques PCR RNA Splicing Termination Western Blotting gene expression genes mRNA molecular biology transcription

Editors and affiliations

  • Jonathan D. Lippiat
    • 1
  1. 1.Institute of Membrane and Systems Biology Faculty of Biological SciencesUniversity of LeedsLeedsUK

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-526-8
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-65-7
  • Online ISBN 978-1-59745-526-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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